Skip to main content

Advertisement

SpringerLink
Log in

Efficacy of extracts and iridoid glucosides from Pentas lanceolata on humoral and cell-mediated immune response of viral vaccine

  • Original Research
  • Published:
Medicinal Chemistry Research Aims and scope Submit manuscript

Abstract

The 80% aqueous methanol extract of Pentas lanceolata (Rubiaceae) was partitioned with n-hexane, ether, ethyl acetate extract, and n-butanol extract. The immunomodulatory role of the five extracts on humoral and cell-mediated immune response of viral vaccine in specific pathogen-free chicken vaccinated against Newcastle disease virus was carried out. The active fractions, n-butanol, was fractioned by silica-column chromatography and active sub-fraction were separated using reverse phase-high performance liquid chromatography. Two new iridoids, namely, 13R-methoxy-epi-gaertneroside 2 and 13S-methoxy-epi-gaertneroside 3, and three known, 13R-epi-gaertneroside 1, a mixture of E-uenfoside 4 and Z-uenfoside 5, were isolated from the active sub-fractions. Their chemical structures were achieved by the extensive 1D and 2D nuclear magnetic resonance (1H and 13C, correlation spectroscopy, heteronuclear multiple quantum correlation, and heteronuclear multiple bond correlation), IR as well as HREIMS spectral data. Stimulation of both seroresponses was observed, especially those of ethyl acetate extract, n-butanol extract, and compound 1. Remarkable effective increase of the antibody titers; lymphocyte and macrophage cells in blood were recorded. The study suggested that the possible role was played by P. lanceolata aerial parts n-butanol, ethyl acetate, and three iridoids 1, 4, and 5 in immunity (humoral and cellular) against Newcastle disease which is severely endangering out the poultry industry by causing economic losses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abd-Alla HI, Moharram FA, Gaara AH, El-Safty MM (2009) Phytoconstituents and immunomodulatory activity of Jatropha curcas L. leaves on humoral and cell-mediated immune response in chicks. Z Naturforsch C 64:495–501

    Article  CAS  PubMed  Google Scholar 

  • Alexander DJ (2000) Newcastle disease and other avian paramyxoviruses. Rev Sci Tech 19:443–462

    Article  CAS  PubMed  Google Scholar 

  • Awad HM, Abd-Alla HI, Mahmoud KH, El-Toumy SA (2014) Invitro anti-nitrosative, antioxidant, and cytotoxicity activities of plant flavonoids: a comparative study. Med Chem Res 23:3298–3307

    Article  CAS  Google Scholar 

  • Bharshiv CK, Garg SK, Bhatia AK (2016) Immunomodulatory activity of aqueous extract of Nyctanthes arbortristis flowers with particularreference to splenocytes proliferation and cytokines induction. Indian J Pharmacol 48:412–417

    Article  PubMed  PubMed Central  Google Scholar 

  • Bremer B, Eriksson T (2009) Time tree of Rubiaceae: phylogeny and dating the family, subfamilies, and tribes. Int J Plant Sci 170:766–793

    Article  Google Scholar 

  • Bukhari SN, Jantan I, Kumolosasi E, Jasamai M (2015) Immunomodulatory effects of diarylpentanoid analogues of curcumin. Med Chem Res 24:1–7

    Article  Google Scholar 

  • Chiang LC, Ng LT, Chiang W, Chang MY, Lin CC (2003) Immunomodulatory activities of flavonoids, monoterpenoids, triterpenoids, iridoid glycosides and phenolic compounds of Plantago species. Planta Med 69:600–604

    Article  CAS  PubMed  Google Scholar 

  • Cimanga K, Hermans N, Apers S, Van Miert S, Van den Heuvel H, Claeys M, Pieters L, Vlietinck A (2003) Complement-inhibiting iridoids from Morinda morindoides. J Nat Prod 66:97–102

    Article  CAS  PubMed  Google Scholar 

  • Dzoyem JP, Donfack AR, Tane P, McGaw LJ, Eloff JN (2016) Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 macrophages and 15-LOX activity by anthraquinones from Pentas schimperi. Planta Med 82:1246–1251

    Article  CAS  PubMed  Google Scholar 

  • Endale M, Alao JP, Akala HM, Rono NK, Eyase FL, Derese S, Ndakala A, Mbugua M, Walsh DS, Sunnerhagen P, Erdelyi M, Yenesew A (2012) Antiplasmodial quinones from Pentas longiflora and Pentas lanceolata. Planta Med 78:31–35

    Article  CAS  PubMed  Google Scholar 

  • Ghisalberti EL (1998) Biological and pharmacological activity of naturally occurring iridoids and secoiridoids. Phytomedicine 5:147–163

    Article  CAS  PubMed  Google Scholar 

  • Hanh NP, Phan NH, Thuan NT, Hanh TT, Vien LT, Thao NP, Thanh NV, Cuong NX, Binh NQ, Nam NH, Kiem PV, Kim YH, Minh CV (2016) Two new simple iridoids from the ant-plant Myrmecodia tuberosa and their antimicrobial effects. Nat Prod Res 30:2071–2076

    Article  CAS  PubMed  Google Scholar 

  • Majiyabe KA, Hitchner SB (1977) Antibody response to strain combinations of Newcastle disease virus as measured by hemagglutination-inhibition (HI) test. Avian Dis 21:576–584

    Article  Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  CAS  PubMed  Google Scholar 

  • Murata K, Abe Y, Futamura-Masuda M, Uwaya A, Isami F, Deng S, Matsuda H (2014) Effect of Morinda citrifolia fruit extract and its iridoid glycosides on blood fluidity. J Nat Med 68:498–504

    Article  CAS  PubMed  Google Scholar 

  • Naranjo DP, Qualls WA, Müller GC, Samson DM, Roque D, Alimi T, Arheart K, Beier JC, Xue RD (2013) Evaluation of boric acid sugar baits against Aedes albopictus (Diptera: Culicidae) in tropical environments. Parasitol Res 112:1583–1587

    Article  PubMed  Google Scholar 

  • Nayak BS, Vinutha B, Geetha B, Sudha B (2005) Experimental evaluation of Pentas lanceolata flowers for wound healing activity in rats. Fitoterapia 76:671–675

    Article  CAS  PubMed  Google Scholar 

  • Ono K, Nakane H, Fukushima M, Chermann JC, Barré-Sinoussi F (1990) Differential inhibitory effects of various flavonoids on the activities of reverse transcriptase and cellular DNA and RNA polymerases. Eur J Biochem 190:469–476

    Article  CAS  PubMed  Google Scholar 

  • Peng W, Liang S, Hu J, Chen Z, Zheng X (2016) The cytotoxic and tyrosine kinase inhibitory properties of steroids and iridoids from the tubers of Alocasia cucullata. J Nat Med 70:602–609

    Article  CAS  PubMed  Google Scholar 

  • Radhakrishnan R, George SK, Kumar S, Balaram P (2014) Maintenance of immunological homeostasis by Indukantha ghritha in patients with recurrent upper respiratory tract infections-a pilot study. Phytother Res 28:1252–1259

    Article  CAS  PubMed  Google Scholar 

  • Richardson MD, Smith H (1981) Resistance of virulent and attenuated strains of Candida albicans to intracellular killing by human and mouse phagocytes. J Infect Dis 144:557–564

    Article  CAS  PubMed  Google Scholar 

  • Robinson MK (1989) Optimization of an in vitro lymphocyte blastogenesis assay for predictive assessment of immunologic responsiveness to contact sensitizers. J Invest Dermatol 92:860–867

    Article  CAS  PubMed  Google Scholar 

  • Schripsema J, Caprini GP, van der Heijden R, Bino R, de Vos R, Dagnino D (2007) Iridoids from Pentas lanceolata. J Nat Prod 70:1495–1498

    Article  CAS  PubMed  Google Scholar 

  • Venditti A, Guarcini L, Ballero M, Bianco A (2015) Iridoid glucosides from Pentas lanceolata (Forssk) Deflers growing on the island of Sardinia. Plant Syst Evol 301:685–690

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to thank Dr. Hisashi Matsuda and Seikou Nakamura from Kyoto Pharmaceutical University; Misasagi, Yamashina-ku, Kyoto 607–8412, Japan for NMR instrumental assistance.

Author information

Authors and Affiliations

  1. Department of Natural Compounds Chemistry, National Research Centre, El-Bohouth Street, Dokki, Giza, 12622, Egypt

    Howaida I. Abd-Alla & Heba-tollah M. Sweelam

  2. Department of Phytochemistry, National Research Centre, El-Bohouth Street, Dokki, Giza, 12622, Egypt

    Tarik A. Mohamed & Mohamed-Elamir F. Hegazy

  3. Department of Botany, Faculty of Science, Cairo University, Cairo, Egypt

    Mahmoud M. Gabr

  4. Central Laboratory for Evaluation of Veterinary Biologics, Abbassia 13181, Cairo, Egypt

    Mounir M. El-Safty

Authors
  1. Howaida I. Abd-Alla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Heba-tollah M. Sweelam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tarik A. Mohamed
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mahmoud M. Gabr
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mounir M. El-Safty
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mohamed-Elamir F. Hegazy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Howaida I. Abd-Alla.

Ethics declarations

Conflicts of interest

The authors declare that they have no competing interests.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abd-Alla, H.I., Sweelam, Ht.M., Mohamed, T.A. et al. Efficacy of extracts and iridoid glucosides from Pentas lanceolata on humoral and cell-mediated immune response of viral vaccine. Med Chem Res 26, 2196–2204 (2017). https://doi.org/10.1007/s00044-017-1935-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00044-017-1935-5

Keywords

Search

Navigation