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Medicinal Chemistry Research

, Volume 26, Issue 10, pp 2336–2344 | Cite as

Beneficial effects of hydroalcoholic extract and flavonoids from Zuccagnia punctata in a rabbit model of vascular dysfunction induced by high cholesterol diet

  • Julieta Roco
  • Gabriela Alarcón
  • Liliana Sierra
  • Iris Catiana Zampini
  • María Ines Isla
  • Susana Jerez
Original Research

Abstract

This study evaluated the effects of a Zuccagnia punctata standardized hydroalcoholic extract (ZpE) and three of its major flavonoids [2′,4′-dihydroxychalcone (DHC), 7-hydroxyflavanone (HF) and 3,7-dihydroxyflavone (DHF)] on the vascular reactivity of aortic rings with endothelial dysfunction induced by feeding rabbits on a high cholesterol diet. Rabbits were fed with either normal chow or a diet containing 1% cholesterol for 5–6 weeks. Isometric contractions were measured. Concentration response curves to ZpE (range from 4 × 10−2 to 4 × 10 µg gallic acid equivalent/ml), DHC, DHF or HF (range from 10−9 to 10−4 M) showed concentration-dependent relaxation of arteries pre-contracted with phenylephrine. ZpE (4 × 10−2, 4 × 10−1 and 4 µg gallic acid equivalent/ml), HF (10−9, 10−7, 10−5 M), DHC (10−9 M) and DHF (10−9 M) added to the bath improved acetylcholine affinity. Pre-treatment of arteries with ZpE (4 × 10−2 µg gallic acid equivalent/ml) and DHC (10−9 M) reduced phenylephrine-induced contraction. Incubation with the higher dose of ZpE (4 µg gallic acid equivalent/ml) reduced the angiotensin II-maximal contraction (C max) acting as a non-competitive antagonist, while DHC and DHF (10−5 M) caused a non-parallel rightward of the angiotensin II-concentration response curves and reduced the C max acting as mixed antagonists. ZpE (4 × 10−2 µg gallic acid equivalent/ml), DHC and DHF (10−9 M) caused a rightward displacement of angiotensin II-concentration response curves acting as competitive antagonists. In conclusion, the present study demonstrated that a ZpE and its major flavonoids had beneficial effects in arteries with vascular dysfunction induced by hypercholesterolemia. Therefore its use as herbal medicine to prevent cardiovascular risks factors may be promising.

Keywords

Zuccagnia punctata Hypercholesterolemia Rabbit aorta Angiotensin II Vascular dysfunction Flavonoids 

Notes

Acknowledgements

This work was supported by grants from the Consejo de Investigaciones de la Universidad Nacional de Tucumán (PIUNT I521/1), Consejo de Investigaciones Científicas y Técnicas de la República Argentina (CONICET PIP 11-232), and Institutional funds from INSIBIO (Instituto Superior de Investigaciones Biológicas). We thank veterinary Rosa Alejandra Molina for bioterio management and Ms Erika Georgieff for her help in data collection.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Instituto Superior de Investigaciones Biológicas (UNT-CONICET)TucumánArgentina
  2. 2.Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán (UNT)TucumánArgentina
  3. 3.Instituto de Bioprospección y Fisiología Vegetal (CONICET-UNT)TucumánArgentina

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