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Medicinal Chemistry Research

, Volume 26, Issue 10, pp 2309–2321 | Cite as

Synthesis, spectral characterization, DNA binding ability and anti-cancer screening of new acridine-based derivatives

  • Othman M. Salem
  • Mária Vilková
  • Jana Janočková
  • Rastislav Jendželovský
  • Peter Fedoročko
  • Ján Imrich
  • Mária Kožurková
Original Research

Abstract

In this study, a series of newly synthesized acridine derivatives, compounds 4, 6a, and 6b, are described and their biological activity on HL-60 cell lines is assessed using a number of different techniques. Binding studies were also performed between the derivatives and DNA in order to characterize the mechanism of the agents’ effect in more detail. The results of ultraviolet–visible absorption spectroscopy prove that the binding of derivatives 4, 6a, and 6b had occurred with a binding constant value of K = 3.5 × 104–4.0 × 104 M−1. These findings are indicative of a strong interaction between the derivatives and DNA, and this hypothesis is supported by the results of the fluorescence emission, linear dichroism, and viscometric assays.

Keywords

Acridine derivatives Topoisomerases I and II HL-60 cells DNA binding 

Notes

Acknowledgements

This study was supported by Slovak Research and Development Agency under contract VVCE-0001-07, VEGA grants No. 1/0001/13, and 1/0672/11 and MH CZ-DRO (UHHK, 00179906). The authors would like to thank Jana Kašpárkova and Viktor Brabec for their advice on LD measurement and viscometric techniques.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Othman M. Salem
    • 1
  • Mária Vilková
    • 2
  • Jana Janočková
    • 1
    • 3
  • Rastislav Jendželovský
    • 4
  • Peter Fedoročko
    • 4
  • Ján Imrich
    • 2
  • Mária Kožurková
    • 1
    • 3
  1. 1.Department of BiochemistryP. J. Šafárik UniversityKošiceSlovakia
  2. 2.Department of Organic Chemistry, Institute of ChemistryP. J. Šafárik UniversityKošiceSlovakia
  3. 3.Biomedical Research CenterUniversity Hospital Hradec KraloveHradec KraloveCzech Republic
  4. 4.Department of Cellular Biology, Faculty of Science, Institute of Biology and EcologyP. J. Šafárik UniversityKošiceSlovakia

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