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Medicinal Chemistry Research

, Volume 26, Issue 7, pp 1506–1515 | Cite as

Novel 2-Arylbenzimidazole derivatives as multi-targeting agents to treat Alzheimer’s disease

  • Oya Unsal-Tan
  • Keriman Ozadali-Sari
  • Beyza Ayazgok
  • Tuba Tüylü Küçükkılınç
  • Ayla Balkan
Original Research

Abstract

This study describes the synthesis, pharmacological evaluation, including acetylcholinesterase (AChE)/butyrylcholinesterase (BChE) inhibition, amyloid beta (Aβ) antiaggregation, and neuroprotective effects, as well as molecular modeling of novel 2-(4-substituted phenyl)-1H-benzimidazole derivatives. These derivatives were synthesized by cyclization of o-phenylenediamines with sodium hydroxy(4-substituted phenyl)methanesulfonate salts. In vitro studies indicated that the most of the target compounds showed remarkable inhibitory activity against BChE (IC50: 13.60–95.44 µM). Among them, 3d and 3g-i also exhibited high selectivity (SI ≥ 35.7) for BChE with IC50 values 39.56, 13.60, 14.45, and 15.15 µM, respectively. According to the molecular modeling studies, it may be assumed that the compounds are able to reach the catalytic site of BChE but not that of AChE. The compounds showing BChE inhibitory effects were subsequently examined for their Aβ-antiaggregating and neuroprotective activities. Among the compounds, 3d inhibited the Aβ1–40 aggregation and demonstrated significant neuroprotection against H2O2-induced and Aβ1–40-induced cell death. Collectively, compound 3d showed the best multifunctional activity (BChE; IC50 = 39.56 µM, SI > 126; Aβ self-mediated aggregation; 67.78% at 100 μM; H2O2-induced cytotoxicity with cell viability of 98% and Aβ1-40-induced cytotoxicity with cell viability of 127%). All these results suggested that 2-(4-(4-methylpiperidin-1-yl)phenyl)-1H-benzo[d]imidazole (compound 3d) could be a promising multi-target lead candidate against Alzheimer’s disease.

Keywords

2-Arylbenzimidazole Alzheimer’s disease Butyrylcholinesterase Aβ aggregation Neuroprotection 

Notes

Acknowledgements

The authors gratefully acknowledge the financial support of the Turkish Scientific Research Institution (TUBITAK, 114S374).

Conflict of interest

The authors declare that they have no conflict of interests.

Supplementary material

44_2017_1874_MOESM1_ESM.doc (2.7 mb)
Supplementary Material

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Oya Unsal-Tan
    • 1
  • Keriman Ozadali-Sari
    • 1
  • Beyza Ayazgok
    • 2
  • Tuba Tüylü Küçükkılınç
    • 2
  • Ayla Balkan
    • 1
  1. 1.Faculty of Pharmacy, Department of Pharmaceutical ChemistryHacettepe UniversityAnkaraTurkey
  2. 2.Faculty of Pharmacy, Department of BiochemistryHacettepe UniversityAnkaraTurkey

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