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Investigation of the interaction between FTO and 3-substituted 2-aminochromones by spectroscopy and molecular modeling

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Abstract

The fat mass and obesity-associated protein is a potential target for anti-obesity medicines. In the present work, the interaction between our synthesized 3-substituted 2-aminochromones and fat mass and obesity-associated protein were investigated using fluorescence spectroscopy, ultraviolet–visible spectroscopy and molecular modeling approach. Fluorescence spectroscopy showed that the fluorescence of fat mass and obesity-associated protein can be quenched by these compounds with a static quenching procedure. In addition, the thermodynamic parameters obtained from the fluorescence data showed that the hydrophobic force played a major role in stabilizing the complexes. Ultraviolet–visible spectroscopy, synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy indicated that these compounds can induce some conformational changes of fat mass and obesity-associated protein.

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Acknowledgements

We are grateful to the National Natural Science Foundation of China (No. 81330075), and 2015 Key science and technology plan project of Henan province (152102310065) for financial support.

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Authors and Affiliations

  1. College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China

    Lijiao Zhang, Zechun Wang, Ting Ren, Hongxu Liu, Xiaona Wang, Ruiyong Wang & Junbiao Chang

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  1. Lijiao Zhang
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  2. Zechun Wang
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  3. Ting Ren
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  4. Hongxu Liu
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  5. Xiaona Wang
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Correspondence to Ruiyong Wang or Junbiao Chang.

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Zhang, L., Wang, Z., Ren, T. et al. Investigation of the interaction between FTO and 3-substituted 2-aminochromones by spectroscopy and molecular modeling. Med Chem Res 26, 1349–1358 (2017). https://doi.org/10.1007/s00044-017-1873-2

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  • DOI: https://doi.org/10.1007/s00044-017-1873-2

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