Abstract
A new series of 4-acetyl-1-substituted-3,4-dihydroquinoxalin-2(1H)-ones (2–13) were designed and synthesized in order to evaluate their AMPA-receptor antagonism as a potential mode of anticonvulsant activity. The structure of the synthesized compounds was confirmed by elemental analysis and spectral data (IR, 1HNMR, 13CNMR and Mass). The molecular design was performed for all the synthesized compounds to predict their binding affinity to AMPA-receptor in order to rationalize their anticonvulsant activity in a qualitative way. The data obtained from the molecular modeling was strongly correlated with that obtained from the biological screening which revealed that; compounds 12b, 13, 12a and 7a showed the highest binding affinities toward AMPA-receptor and also showed the highest anticonvulsant activities against pentylenetetrazole -induced seizures in experimental mice. The relative potencies of these compounds were 1.66, 1.66, 1.61 and 0.82 respectively, in comparing to diazepam.
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Alswah M, Ghiaty A, El-Morsy A, El-Gamal K (2013) Synthesis and biological evaluation of some [1,2,4]triazolo[4,3-a]quinoxaline derivatives as novel anticonvulsant agents. ISRN Org Chem 2013(4):1–7
Baum B, Mohamed M, Zayed M, Gerlach C, Heine A, Hangauer D, Klebe G (2009) More than a simple lipophilic contact: a detailed thermodynamic analysis of non basic residues in the S1 pocket of thrombin. J Mol Biol 390(1):56–69
Bayoumi A, Ghiaty A, El-Morsy A, Abul-Khair H, Hassan MH, Elmeligie S (2012) Synthesis and evaluation of some new 1,2,4-triazolo(4,3-a) quinoxalin-4-5H-one derivatives as AMPA receptor antagonists. Bull Fac Pharm Cairo Univ 50:141–146
Bonuga YR, Nathb AR, Balram B, Ram B (2013) Synthesis and antibacterial activity of 3,4-dihydroquinoxalin-2(1H)-one derivatives. Der PharmaChemica 5(5):296–300.
Catarzi D, Colotta V, Varano F (2007) Competitive AMPA receptor antagonist. Med Res Rev 27(2):239–278
El-Adl K (2011) Design and synthesis of some novel 2-(5-methylbenzoxazol-2-ylsalfanyl)-N-(4-substitutedphenyl)-acetamide derivatives with potential anticonvulsant activity. Al-Azhar J Pharm Sci 44:183–204
El-Helby AA, Ibrahim MK, Abdel-Rahman AA, Ayyad RRA, Menshawy MA, El-Adl K (2009) Synthesis, molecular modeling and anticonvulsant activity of benzoxazole derivatives. Al-Azhar J Pharm Sci 40:252–270
Elhelby AA, Ayyad RR, Zayed MF (2011) Synthesis and biological evaluation of some novel quinoxaline derivatives as anticonvulsant agents. Arzneimittelforschung 61(7):379–381
Elkaeed E, Ghiaty A, El-Morsy A, El-Gamal K, Sak H (2014) Synthesis and biological evaluation of some quinoxaline-2-one derivatives as novel anticonvulsant agents. Chem Sci Rev Lett 3(12):1375–1387
Englert L, Biela A, Zayed M, Heine A, Hangauer D, Klebe G (2010) Displacement of disordered water molecules from hydrophobic pocket creates enthalpic signature: binding of phosphonamidate to the S10-pocket of thermolysin. Biochim Biophys Acta 1800:1192–1202
Faust K, Gehrke S, Yang Y, Yang L, Beal MF, Lu B (2009) Neuroprotective effects of compounds with antioxidant and anti-inflammatory properties in a Drosophila model of Parkinson’s disease. BMC Neurosci 10:109–125
Ibrahim M-K, Abd-Elrahman AA, Ayyad RRA, El-Adl K, Mansour AM, Eissa IH (2013) Design and synthesis of some novel 2-(3-methyl-2-oxoquinoxalin-1(2H)-yl)-N-(4-(substituted)phenyl)-acetamide derivatives for biological evaluation as anticonvulsant agents. Bull Fac Pharm Cairo Univ 51:101–111
Ibrahim M-K, El-Adl K, Zayed MF, Mahdy HA (2015) Design, synthesis, docking, and biological evaluation of some novel 5-chloro-2-substituted sulfanylbenzoxazole derivatives as anticonvulsant agents. Med Chem Res 24(1):99–114
Jin R, Banke TG, Mayer ML, Traynelis SF, Gouaux E (2003) Structural basis for partial agonist action at ionotropic glutamate receptors. Nat Neurosci 6(8):803–810
Jin R, Gouaux E (2003) Probing the function, conformational plasticity, and dimer–dimer contacts of the GluR2 ligand-binding core: studies of 5-substituted willardiines and GluR2 S1S2 in the crystal. Biochemistry 42(18):5201–5213
Loscher W, Rogawski MA. (2002) Epilepsy. In: Lodge D, Danysz W, Parsons CG. (eds) Ionotropic glutamate receptors as therapeutic targets. F.P. Graham Publishing Co, Johnson City, pp 91–132
Rogawski MA (2006) Diverse mechanisms of antiepileptic drugs in the development pipeline. Epilepsy Res 69:273–294
Rogawski MA (2013) AMPA receptors as a molecular target in epilepsy therapy. Acta Neurol Scand Suppl 197:9–18
Traynelis SF, Wollmuth LP, McBain CJ, Menniti FS, Vance KM, Ogden KK, Hansen KB, Yuan H, Myers SJ, Dingledine R (2010) Glutamate receptor ion channels: structure, regulation, and function. Pharmacol Rev 62(3):405–496
Vogel GH (2008) Drug discovery and evaluation: pharmacological assays, Third edition. Springer, New York, p 692–693
Wagle S, Adhikari AV, Kumari NS (2009) Synthesis of some new 4-styryltetrazolo[1,5-a]quinoxaline and 1-substituted-4-styryl[1,2,4] triazolo[4,3-a]quinoxaline derivatives as potent anticonvulsants. Eur J med Chem 44(3):1135–1143
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The authors extend their appreciation and thanking to Prof. Dr. Ahmed M. Mansour, Pharmacology & Toxicology Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt for helping in the pharmacological screening.
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El-Helby, AG.A., Ayyad, R.R.A., El-Adl, K. et al. Design, molecular docking and synthesis of some novel 4-acetyl-1-substituted-3,4-dihydroquinoxalin-2(1H)-one derivatives for anticonvulsant evaluation as AMPA-receptor antagonists. Med Chem Res 25, 3030–3046 (2016). https://doi.org/10.1007/s00044-016-1723-7
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DOI: https://doi.org/10.1007/s00044-016-1723-7