Medicinal Chemistry Research

, Volume 23, Issue 12, pp 5110–5119 | Cite as

Effect of garlic and cabbage on healing of gastric ulcer in experimental rats

  • Taibi Ben HaddaEmail author
  • Naser A. ElSawyEmail author
  • Eslam A. M. Header
  • Yahia N. Mabkhot
  • Mohammad S. Mubarak
Original Research


The effect of garlic (Allium sativum) and cabbage (Brassica oleracea var. capitata) extracts on the healing of gastric ulcer in experimental rats has been investigated. Thirty-three albino male rats (115 ± 4 g B.Wt. each) were used and divided into 6 groups (n = 6 rats); one was used as a negative control while the others were given aspirin orally (200 mg/kg B.Wt.). One of these groups was employed as a positive control and the others were administrated with 150 or 300 mg/kg B.Wt. doses of garlic and cabbage juice for 7 days. The length of the gastric ulcer, the volume of gastric juice, the total acidity, the pH value, the total bacterial count, and the histopathological changes of the stomach were examined. Results revealed that oral administration with both tested plant extracts reduced the length of gastric ulcer, the total acidity, the volume of gastric juice, the bacterial count, and the histopathological changes caused by aspirin. On the other hand, both aqueous plants extract increased the pH value of gastric juice. It is concluded that, garlic and cabbage extract could be used for healing acute gastric ulcer.


Garlic (Allium sativumCabbage (Brassica oleracea var. capitata) juice Aspirin ulcer Histopathological changes pH value Bacterial count Gastric juice 



The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project Number RGP- VPP- 007.


  1. Adeniyi BA, Anyiam FM (2004) In vitro anti-Helicobacter pylori potential of methanol extract of Allium ascalonicum Linn. (Liliaceae) leaf: susceptibility and effect on urease activity. J Phytother Res 18(5):358–361CrossRefGoogle Scholar
  2. Agrawal AK, Rao CV, Sairman K, Joshi VK, Goel RK (2000) Effect of Piper longum linn, Zingiber officianails linn and Ferula species on gastric ulceration and secretion in rats. J Exp Biol 38(10):994–998Google Scholar
  3. Akhtar AH, Ahmed KU (1995) Anti-ulcerogenic evaluation of the methanolic extracts of some indigenous medicinal plants in Pakistan on aspirin-ulcerated rats. J Ethnopharmacol 46(1):1–6CrossRefPubMedGoogle Scholar
  4. Allison MC, Howatson AG, Caroline MG (1992) Gastrointestinal damage associated with the use of nonsteroidal antiinflammatory drugs. N Engl J Med 327:749–754CrossRefPubMedGoogle Scholar
  5. AOAC (1995) Official methods of analysis of the association of official analytical chemists, 16th edn. AOAC International, Washington, p 936Google Scholar
  6. Armitage P, Berry G (1987) Statistical methods in medical research. Black well, Oxford, pp 93–213Google Scholar
  7. Baibekov IM, Mavlian RS (1986) Morphological research on the effect of vagotomy on the parietal micro-flora of the stomach and duodenum. J Biull EKP Biol Med 101(4):496–498Google Scholar
  8. Block E (1985) The chemistry of garlic and onions. J Sci Am 252:114–1192CrossRefGoogle Scholar
  9. Caldwell DR, Danzer CJ (1988) Effects of allyl sulfides on the growth of predominant gut anaerobes. J Curr Microbiol 16:237–241CrossRefGoogle Scholar
  10. Carleton H (1976) Histopathological technique, 4th edn. London. Oxford University Press, New YorkGoogle Scholar
  11. Cellini L, Di Campli E, Masulli M, Di Bartolomeo S, Allocati N (1996) Inhibition of Helicobacter pylori by garlic extracts (Allium sativum). J FEMS Immunol Med Microbiol 13(4):273–277CrossRefGoogle Scholar
  12. Chapman D, Castilla R, Campbell L (1959) Evaluation of protein in food: a method for the determination of feed efficiency ratio. Can J Biochem Phys 37:679–686CrossRefGoogle Scholar
  13. Cheney G (1949) Rapid healing of peptic ulcers in patients receiving fresh cabbage juice. Cal Med 70(1):10–15Google Scholar
  14. Chen-Road H, Su-Lin N (1997) Acid-induced gastric damage in rats is aggravated by starvation and prevented by several nutrients. J Nutr 127(4):630–636Google Scholar
  15. Chung JG, Chen GW, Wu LT (1998) Effects of garlic compounds diallyl sulfide and diallyl disulfide on arylamine N-acetyltransferase activity in strains of Helicobacter pylori from peptic ulcer patients. Am J Chin Med 26:353–364CrossRefPubMedGoogle Scholar
  16. David HA, William FS, Dennis MB (2002) Manual of nutritional therapeutics, 4th edn. Lippincott Williams & Wilkins Comp, London, pp 409–415Google Scholar
  17. Didry N, Dubreuil L, Pinkas M (1992) Antimicrobial activity of naphthoquinones and Allium extracts combined with antibiotics. J Pharm Acta Helv 67(5–6):148–151Google Scholar
  18. Difco’s M (1971) Difco’s Manual of dehydrate culture media and reagent for microbiological and Chemical Laboratoric Procedures. Difco laboratories. Detroit, Michigan, USA Ernst E (1999) Is garlic an effective treatment for Helicobacter pylori infection. J Arch Inter Med 159:2484–2485Google Scholar
  19. Feldberg RS, Chang SC, Kotik AN, Nadler M, Neuwirth Z, Sundstrom DC, Thompson NH (1988) In vitro mechanism of inhibition of bacterial growth by allicin. J Antimicrob Agents Chemother 32:1763–1768CrossRefGoogle Scholar
  20. Fenwick GR, Hanley AB (1985) The genus Allium-part 3. Medicinal effects. CRC Crit Rev Food Sci Nutr J 1:1–74CrossRefGoogle Scholar
  21. Fuchs CS, Mayer RJ (1995) Gastric carcinoma. J Med 333:32–41Google Scholar
  22. Gowsala PS (2001) Protection against Helicobacter pylori and other bacterial infections by garlic. J Nutr 131:1106S–1108SGoogle Scholar
  23. Graham DY, Anderson SY, Lang T (1999) Garlic or jalapeno peppers for treatment of Helicobacter pylori infection. Am J Gastroenterol 94:1200–1202PubMedGoogle Scholar
  24. Hughes BG, Lawson LD (1991) Antimicrobial effects of Allium sativum L. (garlic), Allium ampeloprasum (elephant garlic), and Allium cepa (onion), garlic compounds and commercial garlic supplement products. J Phytother Res 5:154–158CrossRefGoogle Scholar
  25. Jain RC (1993) Antitubercular activity of garlic oil. J Indian Drugs 30:73–75Google Scholar
  26. Jensen DM, You S, Pelayo E (1992) The prevalence of Helicobacter pylori and NSAID use in patients with severe UGI hemorrhage and their potential role in recurrence of ulcer bleeding. J Gastroenterol 102:A90Google Scholar
  27. Jimenez E, Bosch F, Galmes JL, Banos JE (1992) Meta-analysis of efficacy of zinc acexamate in peptic ulcer. Digestion 51:18–26CrossRefPubMedGoogle Scholar
  28. Khosla P, Karan RS, Bhargava VK (2004) Effect of garlic oil on ethanol induced gastric ulcers in rats. J Phytother Res 18(1):87–91CrossRefGoogle Scholar
  29. Lawson DL (1996) The composition and chemistry of garlic cloves and processed garlic. In: Koch HP, Lawson DL (eds) Garlic: the Science and Therapeutic Applications of Allium sativum L. and Related Species, 2nd edn. Williams & Wilkins, Baltimore, pp 37–107Google Scholar
  30. Mahachai V, Thomson AB, Vilaichone RK (2004) Effect of Helicobacter Pylori infection and NSAIDs on the risk of peptic ulcer bleeding. J Med Assoc Thai 87(2):295–299Google Scholar
  31. Mahony R (2005) Bactericidal and anti-adhesive properties of culinary and medicinal plants against Helicobacter pylori. Centre Infect Dis Int Health 11(47):7499–7507Google Scholar
  32. Noess K (1984) Ulcer-fiber-cabbage and vitamin U. Tidsskr Nor Laegeforen 106:693–694Google Scholar
  33. Nostro A (2006) Effects of combining extracts (from propolis or Zingiber officinale) with clarithromycin on Helicobacter pylori. Phytother Res 20(3):187–190CrossRefPubMedGoogle Scholar
  34. Oxoid M (1979) The Oxoid manual of culture media and laboratory services. Forth EditionGoogle Scholar
  35. Patty I, Benedek S, Deak G (1982) Controlled trial of vitamin A therapy in gastric ulcer. Lancet 2(8303):876CrossRefPubMedGoogle Scholar
  36. Patty I, Tarnok F, Simon L (1984) A comparative dynamic study of the effectiveness of gastric cytoprotection by vitamin A, De-Nol, sucralfate and ulcer healing by pirenzepine in patients with chronic gastric ulcer (a multiclinical and randomized study). Acta Physiol Hung 64:379–384PubMedGoogle Scholar
  37. Rees LP, Minney SF, Plummer NT, Slater JH, Skyrme DA (1993) A quantitative assessment of the antimicrobial activity of garlic (Allium sativum). World J Micrbiol Biotechnol 9:303–307CrossRefGoogle Scholar
  38. Reuter HD, Koch HP, Lawson DL (1996) Therapeutic effects and applications of garlic and its preparations. In: Koch HP, Lawson DL (eds) Garlic: the Science and Therapeutic Applications of Alliu sativum L. and Related Species, 2nd edn. Williams & Wilkins, Baltimore, pp 135–212Google Scholar
  39. Shin JE (2005) In vitro inhibitory effect of flavonoids on growth, infection and vacuolation of Helicobacter pylori. J Pharm 71(3):197–201Google Scholar
  40. Sivam GP, Lampe JW, Ulness B, Swanzy SR, Potter JD (1997) Helicobacter pylori in vitro susceptibility to garlic (Allium sativum) extract. J Nutr Cancer 27(2):118–121CrossRefGoogle Scholar
  41. Standard Methods for the Examination of Dairy Products (1960) American health Association pubGoogle Scholar
  42. Suadicani P, Hein HO, Gyntelberg F (1999) Genetic and life-style determinants of peptic ulcer. A study of 3387 men aged 54 to 74 years: The Copenhagen Male Study. Scand J Gastroenterol 34:12–17CrossRefPubMedGoogle Scholar
  43. Thaly H (1965) A new therapy of peptic ulcer: The anti-ulcer factor of cabbage. Gaz Med Fr 72:1992–1993PubMedGoogle Scholar
  44. Uwins C (2006) Growth-induced changes in the proteome of Helicobacter pylori. J Pharm 27(5–6):1136–1146Google Scholar
  45. Walsh JH, Peterson WL (1995) The treatment of Helicobacter pylori infection in the management of peptic ulcer disease. New Engl J Med 333:984–990CrossRefPubMedGoogle Scholar
  46. Wang YC (2005) Screening of anti-Helicobacter pylori herbs deriving from Taiwanese folk medicinal plants. Dep Food Sci 43(2):295–300Google Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Taibi Ben Hadda
    • 1
    Email author
  • Naser A. ElSawy
    • 2
    • 3
    Email author
  • Eslam A. M. Header
    • 4
    • 5
  • Yahia N. Mabkhot
    • 6
  • Mohammad S. Mubarak
    • 7
  1. 1.Laboratoire Chimie Matériaux, FSOUniversité Mohammed 1erOujdaMorocco
  2. 2.Department of Anatomy and Embryology, Faculty of MedicineZagazig UniversityZagazigEgypt
  3. 3.Department of Laboratory Medicine, Faculty of Applied Medical SciencesUmm Al Qura UniversityMakkahSaudi Arabia
  4. 4.Clinical Nutrition, Faculty of Applied Medical SciencesUmm Al Qura UniversityMakkahSaudi Arabia
  5. 5.Department of Nutrition and Food Science, Faculty of Home EconomicsMinufiya UniversityShebin El KomEgypt
  6. 6.Department of Chemistry, Faculty of ScienceKing Saud UniversityRiyadhSaudi Arabia
  7. 7.Department of ChemistryThe University of JordanAmmanJordan

Personalised recommendations