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Design, synthesis and docking studies of 2-benzoxazolinone-based 1,2,4-triazoles as proinflammatory cytokine inhibitors

Abstract

A library of 20 novel benzoxazolinone-based 1,2,4-triazoles has been synthesised and screened for their in vivo anti-inflammatory and antinociceptive activities. The compound 18e exhibited potent anti-inflammatory activity with 68.75 and 55.20 % inhibition in comparison to indomethacin which showed 65.62 and 60.41 % inhibition after 3 and 5 h, respectively. The five active compounds, i.e. 2a and 17e20e showing significant in vivo anti-inflammatory activity have been screened for their in vivo COX-2, TNF-α, IL-1β and NO inhibitory activities. The COX-2 selectivity index of 17e was found to be 42.30 and thereby showing a high selectivity towards COX-2 inhibition. In silico molecular docking studies have been done in order to get an insight into the binding modes of these molecules with TNF-α protein. The compound 20e exhibited potent antinociceptive activity with 56.70 % inhibition of analgesia. The compounds 2a and 17e20e did not exhibit any gastric ulceration.

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Acknowledgments

The authors thank Dr. G. N. Qazi, Vice-Chancellor, Jamia Hamdard for providing necessary facilities to the Department of Chemistry. One of the authors (SH) is also thankful to CSIR for awarding the senior research fellowship.

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The authors report no conflict of interest.

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Correspondence to Mohammad Sarwar Alam.

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Haider, S., Alam, M.S., Hamid, H. et al. Design, synthesis and docking studies of 2-benzoxazolinone-based 1,2,4-triazoles as proinflammatory cytokine inhibitors. Med Chem Res 23, 4250–4268 (2014). https://doi.org/10.1007/s00044-014-0989-x

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Keywords

  • Cyclooxygenase
  • Molecular docking
  • Antinociceptive
  • 2AZ5 ligand