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Synthesis, molecular docking of novel 1,8-naphthyridine derivatives and their cytotoxic activity against HepG2 cell lines

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Abstract

A series of novel 2,7-dimethyl-1,8-naphthyridine derivatives substituted with Mannich bases 2ad, N-β-glycosides 6ae, 7ae, Schiff’s bases 8ac, pyrazolone 9, and S-alkylated derivatives 10ac have been synthesized in good yields starting from 4-hydroxy-2,7-dimethyl-1,8-naphthyridine 1 through multi-step synthesis. The newly synthesized title compounds were evaluated for their HepG2 cell growth inhibition, the results revealed that all the tested compounds process inhibitory effects on the growth of HepG2 liver cancer cells. Compound 8b showed the highest inhibition activity against HepG2 cell line (IC50 equals 3.2 μg/mL) among all tested compounds. The results were compared to 5-Fluorouracil (5-FU) and doxorubicin as reference drugs, (IC50 5 and 3.56 μg/mL). Furthermore, molecular docking of compounds 3b, 6a, and 8b into the binding site of topoisomerase II was carried out. The results of the binding energy scores of these compounds were compared to the docking score of Vosaroxin, a known 1,8-naphthyridine derivative which is in clinical trials as potential anticancer drug. Compound 8b docking result revealed that it is the only tested compound that intercalate with DNA segment of the topoisomerase II, similar to Vosaroxin which was used as reference drug for docking comparison.

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Correspondence to Ahmad F. Eweas.

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Eweas, A.F., Khalifa, N.M., Ismail, N.S. et al. Synthesis, molecular docking of novel 1,8-naphthyridine derivatives and their cytotoxic activity against HepG2 cell lines. Med Chem Res 23, 76–86 (2014). https://doi.org/10.1007/s00044-013-0604-6

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  • DOI: https://doi.org/10.1007/s00044-013-0604-6

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