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Medicinal Chemistry Research

, Volume 22, Issue 9, pp 4347–4359 | Cite as

Bioactivity guided isolation of antiinflammatory, analgesic, and antipyretic constituents from the leaves of Pedilanthus tithymaloides (L.)

  • Soma Ghosh
  • Debprasad Chattopadhyay
  • Anurup Mandal
  • Sudipta Kaity
  • Amalesh SamantaEmail author
Original Research

Abstract

Pedilanthus tithymaloides (PT) leaves are widely used in Indian medicine to treat inflammation and pain, but until recently no systematic study of these activities were reported. This study aimed to evaluate the antiinflammatory, antinociceptive, and antipyretic activity of the chloroform (CE) and methanol extract (ME) of PT leaves and its isolated constituents in animal models. The results revealed significant antiinflammatory activity of CE and ME in carrageenan-induced paw edema (acute), vascular permeability and cotton pellet granuloma (chronic) models. Both extracts also produced significant increase in hot-plate reaction time but decrease in writhing response in a dose-dependent manner, indicating their antinociceptive activity. Moreover, significant antipyretic activity was recorded in yeast-induced pyrexia model. Chemical study of CE and ME yielded five known compounds, namely epifriedelanyl acetate (1), friedelanol (2), β-sitosterol (3), ursolic acid (4), and luteolin (5), along with the new compound 1,2-tetradecanediol 1-(hydrogen sulfate) sodium salt (6). This is the first report showing isolation of compound 5 from PT leaves, and of compound 6, from any plant source. Among these, compounds 4 and 5 (10 mg/kg, p.o.) produced significant inhibition of pain response elicited by acetic acid, increased latency in hot-plate, and caused inhibition of carrageenan-induced edema and cotton pellet-induced granuloma formation in mice. Thus, our results clearly demonstrated the justification of the traditional use of P. tithymaloides leaves as an antiinflammatory drug in primary health care.

Keywords

Pedilanthus tithymaloides Antiinflammatory Carrageenan-induced paw edema Antinociceptive Antipyretic 

Notes

Acknowledgments

The authors are grateful to the Indian Council of Medical Research, New Delhi for providing Senior Research Fellowship to one of the authors (SG), and the Department of Pharmaceutical Technology, Jadavpur University, Kolkata for providing all the necessary facilities. Authors are also thankful to the Scientists of Division of Natural Product Chemistry, Indian Institute of Chemical Biology, Jadavpur, Kolkata, for their help in isolation and spectral data interpretation.

Supplementary material

44_2012_449_MOESM1_ESM.docx (3.3 mb)
Supplementary material 1 (DOCX 3386 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Soma Ghosh
    • 1
  • Debprasad Chattopadhyay
    • 2
  • Anurup Mandal
    • 1
  • Sudipta Kaity
    • 1
  • Amalesh Samanta
    • 1
    Email author
  1. 1.Division of Microbiology, Department of Pharmaceutical TechnologyJadavpur UniversityKolkataIndia
  2. 2.ICMR Virus UnitI.D and B.G Hospital KolkataIndia

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