Abstract
Indian traditional medicinal plant Piper attenuatum (Buch-Ham) has been investigated for its antioxidant and anticancer activity. Three extracts were prepared using ethyl acetate, ethanol and methanol. In vitro antioxidant activity was performed by ABTS {2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)} free radical scavenging method. All three extracts reduced the free radicals produced by ABTS in a concentration-dependent manner which could be compared to the standard (gallic acid). Invitro anticancer activity of all extracts was carried out by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay method against MCF7 (Michigan Cancer Foundation-7) cell lines. None of the extract showed anticancer activity when compared with the standard (mitomycin C) indicating that P. attenuatum is deprived of antiproliferative or cytotoxic components.
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Acknowledgments
We acknowledge Dr. C. V. Chandrashekhar, Natural remedies, Bengaluru, for helping us in carrying biological activity studies. We also acknowledge Dr. M. M. Rao, The Director, NIAPR, Patiala, for providing the laboratory facilities for this study.
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Renu Ohlyan and Ajit Kandale contributed equally to this study.
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Ohlyan, R., Kandale, A., Deora, G.S. et al. In vitro screening of dry fruit extracts of Piper attenuatum for antioxidant and anticancer activity. Med Chem Res 22, 1365–1370 (2013). https://doi.org/10.1007/s00044-012-0122-y
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DOI: https://doi.org/10.1007/s00044-012-0122-y