Skip to main content

Anti-inflammatory and antioxidant activities of some extracts and pure natural products isolated from Rhus tripartitum (Ucria)


Chromatographic separation of Rhus tripartitum (Anacardiaceae) chemical components revealed the presence of four main natural substances: a new biflavonoid masazinoflavanone (S1), myricetin (S2), (-)-lyoniresinol 3α-O-β-D-glucopyranoside (S3), and (-)-methyl shikimate (S4). The anti-inflammatory activity of the new biflavonoid substance was performed. Compared with the standard drug dexamethasone, the biflavonoid substance masazinoflavanone (S1) inhibited in a dose-related manner carrageenan-induced paw edema in rats. Antiradical scavenging capacities of extracts and natural substances issued from Rhus tripartitum were tested using free radical forms of ABTS and DPPH. The Trolox Equivalent Antioxidant Capacity (TEAC) assay shows values of 1.92, 1.39, 1.25, 2.40, and 1.58 mM Trolox/mg sample respectively for the chloroformic extract, the ethyl acetate extract, (S1), (S2), and (S3). Values revealed form the total phenolic content assay were 299.3, 372.3, 99.1, and 235.7 mg Catechin/g extract respectively for the chloroformic, ethyl acetate, ethyl acetate/methanol, and methanolic extracts.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3


  1. Ammar S, Mahjoub MA, Charfi N, Skandarani I, Chekir-Ghedira L, Mighri Z (2007) Mutagenic, antimutagenic and antioxidant activities of a new class of β-glucoside hydroxyhydroquinone from Anagallis monelli growing in Tunisia. Chem Pharm Bull 55:385–388

    Article  CAS  PubMed  Google Scholar 

  2. Bel Hadj Salah K, Mahjoub MA, Chaumont JP, Michel L, Millet-Clerc J, Cherif I, Ammar S, Mighri Z, Aouni M (2006) Antimicrobial and antioxidant activity of the methanolic extracts of three Salvia species from Tunisia. Nat Prod Res 20:1110–1120

    Article  Google Scholar 

  3. Bilici D, Akpinar E, Kiziltuç A (2002) Protective effect of melatonin in carrageenan-induced acute local inflammation. Pharmacological Res 46:133–139

    Article  CAS  Google Scholar 

  4. Braham H, Mighri Z, Ben Jannet H, Matthew S, Abreu P (2005) Antioxidant phenolic glycosides from Moricandia arvensis. J Nat Prod 68:517–522

    Article  CAS  PubMed  Google Scholar 

  5. Hatano T, Kagawa H, Yasuhara T, Okuda T (1988) Two new flavonoids and other constituents in licorice root; their relative astringency and radical scavenging effects. Chem Pharm Bull 36:2090–2097

    CAS  PubMed  Google Scholar 

  6. Le Floc’h E (1983) Contribution à une étude ethnobotanique de la Flore Tunisienne. Tunis, Tunisian Scientific Publications: Official Printing Works of the Tunisian Republic

  7. Mahjoub MA, Ben Jannet H, Abreu P, Mighri Z (2002) Etudes biologique et chimique de la plante Prasium majus poussant en Tunisie structure d’un hétéroside stéroïdique et d’un stéroïde, identification de constituants de la fraction volatile. J Soc Chim de Tunisie 4:1419–1426

    CAS  Google Scholar 

  8. Mahjoub MA, Ammar S, Mighri Z (2004) Isolation and structure studies of a few natural compounds from the Tunisian plant Rhus tripartitum. J Soc Alger Chim 14:293–297

    CAS  Google Scholar 

  9. Miller NJ, Rice-Evans CA, Davies MJ, Gopinathan V, Milner A (1993) A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84:407–412

    CAS  PubMed  Google Scholar 

  10. Pottier Alapetite G (1981) Flore de la Tunisie. Tunis, Tunisian Scientific Publications

    Google Scholar 

  11. Prokai L, Simpkins JW (2007) Structure–nongenomic neuroprotection relationship of estrogens and estrogen-derived compounds. Pharmacol Therapeut 114:1–12

    Article  CAS  Google Scholar 

  12. Van den Berg R, Haenen Guido RMM, van den Berg H, Bas A (1999) Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem 66:511–517

    Article  Google Scholar 

  13. Velioglu YS, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agr Food Chem 46:4113–4117

    Article  CAS  Google Scholar 

  14. Winter CA, Risley GA, Nuss GW (1962) Carrageenan-induced oedema in hind paws of the rat as an assay for anti-inflammatory drugs. Pro Soc Exp Bio Med 3:544–547

    Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Mohamed Ali Mahjoub.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mahjoub, M.A., Ammar, S., Edziri, H. et al. Anti-inflammatory and antioxidant activities of some extracts and pure natural products isolated from Rhus tripartitum (Ucria). Med Chem Res 19, 271–282 (2010).

Download citation


  • Rhus tripartitum
  • New biflavonoid
  • Anti-inflammatory activity
  • Antioxidant activity
  • Total phenolic content