Insectes Sociaux

, Volume 62, Issue 2, pp 227–236 | Cite as

When is the best period to sample ants in tropical areas impacted by mining and in rehabilitation process?

  • A. M. RabelloEmail author
  • A. C. M. Queiroz
  • C. J. Lasmar
  • R. G. Cuissi
  • E. O. Canedo-Júnior
  • F. A. Schmidt
  • C. R. Ribas
Research Article


Fluctuating resources and conditions can regulate the community structure of ants, affecting their activities and interactions. This has important implications for the selection of sampling periods during bioindication studies. The aim of this study was to evaluate if the chosen sampling period influences the response of ant assemblage diversity and ecological function to mining impacts and rehabilitation age after mining. We used ant assemblage parameters, including arboreal, epigaeic and hypogaeic ant species richness and composition, seed-removing ant species richness and composition, and seed removal rate in areas impacted by mining and with different rehabilitation ages. We showed that in most cases the response is the same, regardless of sampling period. However, we suggest that ant sampling is best undertaken in the rainy period, when ant species richness reaches higher values and the assemblage composition presents a well-marked difference among the areas. We also indicate that the epigaeic ant assemblage is sufficient for evaluating mining impacts, but arboreal strata should also be sampled in rehabilitation scenarios.


Bioindication Cerrado Formicidae Assemblage diversity Seed removal 



This work resulted from the research project: FAPEMIG—CRA—RDP–00123-10—“Biodiversidade e funções ecológicas de formigas—bioindicação de impactos ambientais e de recuperação de áreas degradadas”. We are thankful to Vale S.A. who permitted sampling in its mining and rehabilitation sites, to Ramon Braga and Cássio Mendanha for helping to choose the mining sites for the study, and Anderson Matos for helping us inside the sites. We thank the Chemical, Biochemical and Food Analyses Laboratory (Food Engineering Department, Federal University of Lavras) for providing a space to produce the artificial fruits. We are grateful to colleagues at LEF: E.A. Silva, T. Moretti and F. Tanure for helping us with fieldwork. We also thank R. Feitosa and D. Braga for verifying ant species identification. We are also thankful to Ross Thomas for revising the English, and the anonymous reviewers who improved this manuscript. The authors received grants from CAPES, CNPq and FAPEMIG.


  1. Andersen AN (2008) Not enough niches: non-equilibrial processes promoting species coexistence in diverse ant communities. Aust Ecol 33:211–220CrossRefGoogle Scholar
  2. Barrow L, Parr C (2008) A preliminary investigation of temporal patterns in semiarid ant communities: variation with habitat type. Aust Ecol 33:653–662CrossRefGoogle Scholar
  3. Basu P (1997) Seasonal and spatial patterns in ground foraging ants in a rain forest in the Western Ghats, India. Biotropica 29:489–500CrossRefGoogle Scholar
  4. Batalha MA, Aragaki S, Mantovani W (1997) Variações fenológicas das espécies do cerrado em Emas (Pirassununga, SP). Acta Bot Bras 11:61–78Google Scholar
  5. Bates D, Maechler M, Bolker B, Walker S (2013) lme4 Linear mixed-effects models using Eigen and S4. R package version 1Google Scholar
  6. Berghoff SM, Weissflog A, Linsenmair KE, Hashim R, Maschwitz U (2002) Foraging of a hypogaeic army ant: a long neglected majority. Insectes Soc 49:133–141CrossRefGoogle Scholar
  7. Bolton B (1994) Identification guide to the ant genera of the world. Harvard University Press, CambridgeGoogle Scholar
  8. Brandão CRF, Silva RR, Delabie JHC (2012) Neotropical ants (Hymenoptera) functional groups: Nutritional and applied implications. In: Panizzi AR, Panizzi JRP (Orgs.) Insect bioecology and nutrition for integrated pest management. CRS Press, Boca Raton, pp 213–236Google Scholar
  9. Campos RI, Vasconcelos HL, Ribeiro SP, Neves FS, Soares JP (2006) Relationship between tree size and insect assemblages associated with Anadenanthera macrocarpa. Ecography 29:442–450CrossRefGoogle Scholar
  10. Christianini AV, Oliveira PS (2010) Birds and ants provide complementary seed dispersal in a neotropical savanna. J Ecol 98:573–582CrossRefGoogle Scholar
  11. Clarke KR, Gorley RN (2006) Primer v6: user manual/tutorial. Plymouth: Plymouth Marine Laboratory, 2006Google Scholar
  12. Coelho IR, Ribeiro SP (2006) Environment heterogeneity and seasonal effects in ground-dwelling ant (Hymenoptera: Formicidae) assemblages in the Parque Estadual do Rio Doce, MG, Brazil. Neotropical Entomol 35:19–29CrossRefGoogle Scholar
  13. Cook SC, Eubanks MD, Gold RE, Behmer ST (2011) Seasonality directs contrasting food collection behavior and nutrient regulation strategies in ants. PLoS One 6:e25407CrossRefPubMedCentralPubMedGoogle Scholar
  14. Dias ATC, Bozelli RL, Darigo RM, Esteves FA, dos Santos HF, Figueiredo-Barros MP, Nunes MFQS, Roland F, Zamith LR, Scarano FR (2012) Rehabilitation of a bauxite tailing substrate in Central Amazonia: the effect of litter and seed addition on flood prone forest restoration. Restor Ecol 20:483–489CrossRefGoogle Scholar
  15. Dominguez-Haydar Y, Armbrecht I (2011) Response of ants and their seed removal in rehabilitation areas and Forests at El Cerrejón Coal Mine in Colombia. Restor Ecol 19:178–184CrossRefGoogle Scholar
  16. Fornara DA, Dalling JW (2005) Post-dispersal removal of seeds of pioneer species from five Panamanian forests. J Trop Ecol 21:79–84CrossRefGoogle Scholar
  17. Grimbacher PS, Stork NE (2009) Seasonality of a diverse beetle assemblage inhabiting lowland tropical rain forest in Australia. Biotropica 41:328–337CrossRefGoogle Scholar
  18. Hahn AD, Wheeler DE (2002) Seasonal foraging activity and bait preferences of ants on Barro Colorado Island, Panama. Biotropica 34:348–356CrossRefGoogle Scholar
  19. Heller NE, Sanders NJ, Shors JW, Gordon DM (2008) Rainfall facilitates the spread, and time alters the impact, of the invasive Argentine ant. Oecologia 155:385–395CrossRefPubMedGoogle Scholar
  20. Herbers JM (1985) Seasonal structuring of a north temperate ant community. Insectes Soc 32:224–240CrossRefGoogle Scholar
  21. Hollec M, Frouz J (2005) Ant (Hymenoptera: Formicidae) communities in reclaimed and unreclaimed brown coal mining spoil dumps in the Czech Republic. Pedobiologia 49:345–357CrossRefGoogle Scholar
  22. Kaspari M, Weiser MD (2000) Ant activity along moisture gradients in a Neotropical forest. Biotropica 32:703–711CrossRefGoogle Scholar
  23. Kaspari M, Yuan M, Alonso L (2003) Spatial grain and the causes of regional diversity gradients in ants. Am Nat 161:459–477CrossRefPubMedGoogle Scholar
  24. Keroumi AE, Naamani K, Soummane H, Dahbi A (2012) Seasonal dynamics of ant community structure in the Moroccan Argan Forest. J Ins Sci 12:94Google Scholar
  25. Leal IR, Oliveira PS (2000) Foraging ecology of attine ants in a Neotropical savanna: seasonal use of fungal substrate in the cerrado vegetation of Brazil. Insectes Soc 47:376–382CrossRefGoogle Scholar
  26. Levings SC (1983) Seasonal, annual and among site variation in the ground ant community of a deciduous tropical forest: some causes of patchy species distributions. Ecol Mono 53:435–455CrossRefGoogle Scholar
  27. Madeira BG, Espírito-Santo MM, D´Ângelo-Neto S, Nunes YRF, Sánchez-Azofeifa GA, Fernandes GW, Quesada M (2009) Changes in tree and liana communities along a successional gradient in a tropical dry forest in southeastern Brazil. Plant Ecol 201:291–304CrossRefGoogle Scholar
  28. Majer JD (1983) Ants: bio-indicators of minesite rehabilitation, land-use, and land conservation. Environ Manag 7:375–383CrossRefGoogle Scholar
  29. Majer JD, Day JE, Kabay ED, Perriman WS (1984) Recolonization by ants in bauxite mines rehabilitated by a number of different methods. J Appl Ecol 21:355–375CrossRefGoogle Scholar
  30. Majer JD, Brennan KEC, Moir ML (2007) Invertebrates and the restoration of a forest ecosystem: 30 years of research following bauxite mining in Western Australia. Restor Ecol 15:S104–S115CrossRefGoogle Scholar
  31. Neves FS, Braga RF, Espírito-Santo MM, Belabie JHC, Fernandes GW, Sánchez-Azofeifa GA (2010) Diversity of arboreal ants in a Brazilian tropical dry forest: effects of seasonality and successional stage. Sociobiology 56:177–194Google Scholar
  32. Neves FS, Queiroz-Dantas KS, Da Rocha WD, Delabie JHC (2013) Ants and three adjacent habitats of a transition region between the Cerrado and Caatinga biomes: the effects of heterogeneity and variation in canopy cover. Neotropical Entomol 42:258–268CrossRefGoogle Scholar
  33. Ottonetti L, Tucci L, Santini G (2006) Recolonization patterns of ants in a rehabilitated lignite mine in Central Italy: potential for the use of mediterranean ants as indicators of restoration processes. Restor Ecol 14:60–66CrossRefGoogle Scholar
  34. Palacio EE, Fernandéz F (2003) Claves para las subfamílias y géneros. In: Fernandéz F (ed) Introducción a las hormigas de la región neotropical. Instituto Humboldt, Bogotá, p 424Google Scholar
  35. Philpott SM, Perfecto I, Vandermeer J (2006) Effects of management intensity and season on arboreal and diversity and abundance in coffee agroecosystems. Biodivers Cons 15:125–141Google Scholar
  36. Philpott SM, Perfecto I, Armbrecht I, Parr CL (2010) Ant diversity and function in disturbed and changing habitats. In: Lach L, Parr CL, Abbott KL (eds) Ant Ecology. Oxford, Oxford, pp 137–142Google Scholar
  37. Pizo MA, Oliveira PS (2001) Size and lipid content of nonmyrmecochorous diaspores: effects on the interaction with litter foraging ants in the Atlantic Rain Forest of Brazil. Plant Ecol 157:37–52CrossRefGoogle Scholar
  38. R Development Core Team (2013) R: A language and environment for statistical computing. R Foundation for Statistical computing, Vienna, Austria.
  39. Raimundo RLG, Guimarães PR Jr, Almeida-Neto M, Pizo MA (2004) The influence of fruit morphology and habitat structure on ant-seed interactions: a study with artificial fruits. Sociobiology 44:261–270Google Scholar
  40. Read JL (1996) Use of ants to monitor environmental impacts of salt spray from a mine in arid Australia. Biodiver Cons 5:1533–1543CrossRefGoogle Scholar
  41. Retana J, Cerdá X (2000) Patterns of diversity and composition of Mediterranean ground ant communities tracking spatial and temporal variability in the thermal environmental. Oecologia 123:436–444CrossRefGoogle Scholar
  42. Ribas CR, Schmidt FA, Solar RRC, Campos RBF, Valentim CL, Schoereder JH (2012a) Ants as indicators of the success of rehabilitation efforts in deposits of gold mining tailings. Restor Ecol 20:712–720CrossRefGoogle Scholar
  43. Ribas CR, Campos RBF, Schmidt FA, Solar RRC (2012b) Ants as indicators in Brazil: a review with suggestions to improve the use of ants in environmental monitoring programs. Psyche 2012:1–23CrossRefGoogle Scholar
  44. Ribas CR, Solar RRC, Campos RBF, Schmidt FA, Valentim CL, Schoereder JH (2012c) Can ants be used as indicators of environmental impacts caused by arsenic? J Ins Cons 16:413–421CrossRefGoogle Scholar
  45. Rico-Gray V, Gracia-Franco JG, Palacios-Rios M, Diar-Castelazo C, Parra-Tabla V, Navarro JA (1998) Geographical and seasonal variation in the richness of ant-plant interactions in Mexico. Biotropica 30:190–200CrossRefGoogle Scholar
  46. Rico-Gray V, Oliveira PS, Parra-Tabla V, Cuautle M, Díaz-Castelazo C (2004) Ant-plant interactions: their seasonal variation and effects on plant fitness. In: Martínez ML, Psuty N, Lubke R (eds) Coastal sand dunes: ecology and restoration. Springer, Berlin Heidelberg, pp 221–239Google Scholar
  47. Schmidt FA, Solar RRC (2010) Hypogeic pitfall traps: methodological advances and remarks to improve the sampling of a hidden ant fauna. Insectes Soc 57:261–266CrossRefGoogle Scholar
  48. Schmidt FA, Ribas CR, Schoereder JH (2013) How predictable is the response of ant assemblages to natural forest recovery? Ecol Indicators 24:158–166CrossRefGoogle Scholar
  49. So WY, Chu LM (2010) Ant assemblages on rehabilitated tropical landfills. Biodiver Cons 19:3685–3697CrossRefGoogle Scholar
  50. Sobrinho TG, Schoereder JH, Sperber CF, Madureira MS (2003) Does fragmentation alter species composition in ant communities (Hymenoptera: Formicidae)? Sociobiology 42:329–342Google Scholar
  51. van Hamburg H, Andersen AN, Meyer WJ, Robertson HG (2004) Ant community development on rehabilitated ash dams in the South African Highveld. Restor Ecol 12:552–558CrossRefGoogle Scholar
  52. Viana-Silva FEC, Jacobi CM (2012) Myrmecofauna of ironstone outcrops: composition and diversity. Neotropical Entomol 41:263–271CrossRefGoogle Scholar
  53. Warren RJ, Giladi I, Bradford MA (2012) Environmental heterogeneity and interspecific interactions influence nest occupancy by key seed-dispersing ants. Environ Entomol 41:463–468CrossRefPubMedGoogle Scholar
  54. Wolda H (1978) Seasonal fluctuations in rainfall, food and abundance of tropical insects. J Anim Ecol 47:369–381CrossRefGoogle Scholar
  55. Wolda H (1988) Insect seasonality: why? AnnRevEcol Syst 19:1–18Google Scholar
  56. Wolda H (1989) Seasonal cues in tropic organisms. Rainfall? Not necessarily! Oecologia 80:437–442CrossRefGoogle Scholar

Copyright information

© International Union for the Study of Social Insects (IUSSI) 2015

Authors and Affiliations

  • A. M. Rabello
    • 1
    Email author
  • A. C. M. Queiroz
    • 1
  • C. J. Lasmar
    • 1
  • R. G. Cuissi
    • 1
  • E. O. Canedo-Júnior
    • 2
  • F. A. Schmidt
    • 3
  • C. R. Ribas
    • 4
  1. 1.Programa de Pós-Graduação em Ecologia Aplicada, Departamento de Biologia, Setor de Ecologia e Conservação, Laboratório de Ecologia de FormigasUniversidade Federal de LavrasLavrasBrazil
  2. 2.Programa de Pós-Graduação em Entomologia, Departamento de Entomologia, Laboratório de Ecologia de FormigasUniversidade Federal de LavrasLavrasBrazil
  3. 3.Centro de Ciências Biológicas e da NaturezaUniversidade Federal do AcreRio BrancoBrazil
  4. 4.Departamento de Biologia, Setor de Ecologia e Conservação, Laboratório de Ecologia de FormigasUniversidade Federal de LavrasLavrasBrazil

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