Insectes Sociaux

, Volume 60, Issue 1, pp 65–73 | Cite as

The effects of honey bee (Apis mellifera L.) queen reproductive potential on colony growth

Research Article

Abstract

Reproduction in species of eusocial insects is monopolized by one or a few individuals, while the remaining colony tasks are performed by the worker caste. This reproductive division of labor is exemplified by honey bees (Apis mellifera L.), in which a single, polyandrous queen is the sole colony member that lays fertilized eggs. Previous work has revealed that the developmental fate of honey bee queens is highly plastic, with queens raised from younger worker larvae exhibiting higher measures in several aspects of reproductive potential compared to queens raised from older worker larvae. Here, we investigated the effects of queen reproductive potential (“quality”) on the growth and winter survival of newly established honey bee colonies. We did so by comparing the growth of colonies headed by “high-quality” queens (i.e., those raised from young worker larvae, which are more queen-like morphologically) to those headed by “low-quality” queens (i.e., those raised from older worker larvae, which are more worker-like morphologically). We confirmed that queens reared from young worker larvae were significantly larger in size than queens reared from old worker larvae. We also found a significant positive effect of queen grafting age on a colony’s production of worker comb, drone comb, and stored food (honey and pollen), although we did not find a statistically significant difference in the production of worker and drone brood, worker population, and colony weight. Our results provide evidence that in honey bees, queen developmental plasticity influences several important measures of colony fitness. Thus, the present study supports the idea that a honey bee colony can be viewed (at least in part) as the expanded phenotype of its queen, and thus selection acting predominantly at the colony level can be congruent with that at the individual level.

Keywords

Apis mellifera Colony growth Expanded phenotype Honey bees Reproduction Queen quality 

Supplementary material

40_2012_267_MOESM1_ESM.xlsx (43 kb)
Supplementary material 1 (XLSX 42 kb)

References

  1. Barchuk A.R., Cristino A.S., Kucharski R., Costa L.F., Simoes Z.L.P. and Maleszka R. 2007. Molecular determinants of caste differentiation in the highly eusocial honeybee Apis mellifera. BMC Devel. Biol. 7: 70Google Scholar
  2. Collins A.M. and Donoghue A.M. 1999. Viability assessment of honey bee, Apis mellifera sperm using dual fluorescent staining. Theriogenology 51: 1513–1523Google Scholar
  3. Cook V.A. 1968. Severe clipping of queens’ wings increases supersedure rate. N. Z. Beekpr. 30: 32Google Scholar
  4. Crozier R.H. and Pamilo P. 1996. Evolution of Social Insect Colonies: Sex Allocation and Kin Selection. Oxford University Press, New York, NYGoogle Scholar
  5. de Azevedo S.V. and Hartfelder K. 2008. The insulin signaling pathway in honey bee (Apis mellifera) caste development - differential expression of insulin-like peptides and insulin receptors in queen and worker larvae. J. Insect Physiol. 54: 1064–1071Google Scholar
  6. Dedej S., Hartfelder K., Aumeier P., Rosenkranz P. and Engels W. 1998. Caste determination is a sequential process: effect of larval age at grafting on ovariole number, hind leg size and cephalic volatiles in the honey bee (Apis mellifera carnica). J. Apic. Res. 37: 183–190Google Scholar
  7. Delaney D.A., Keller J.J., Caren J.R. and Tarpy D.R. 2011. The physical, insemination, and reproductive quality of honey bee queens (Apis mellifera L.). Apidologie 42: 1–13Google Scholar
  8. Eckert J.E. 1934. Studies in the number of ovarioles in queen honeybees in relation to body size. J. Econ. Entomol. 27: 629–635Google Scholar
  9. Farrar C.L. 1947. Nosema losses in package bees as related to queen supersedure and honey yields. J. Econ. Entomol. 40: 333–338Google Scholar
  10. Fjerdingstad E.J. and Boomsma J.J. 1997. Variation in size and sperm content of sexuals in the leafcutter ant Atta colombica. Insect. Soc. 44: 209–218Google Scholar
  11. Fjerdingstad E.J. and Boomsma J.J. 1998. Multiple mating increases the sperm stores of Atta colombica leafcutter ant queens. Behav. Ecol. Sociobiol. 42: 257–261Google Scholar
  12. Fjerdingstad E.J. and Keller L. 2004. Relationships between phenotype, mating behavior, and fitness of queens in the ant Lasius niger. Evolution 58: 1056–1063Google Scholar
  13. Frazier M.A., Mullin C., Frazier J. and Ashcraft S. 2008. What have pesticides got to do with it? Am. Bee J. 148: 521–523Google Scholar
  14. Furgala B. 1962. The effect of the intensity of Nosema inoculum on queen supersedure in the honey bee, Apis mellifera Linnaeus. J. Insect Pathol. 4: 429–432Google Scholar
  15. Gilley D.C., Tarpy D.R. and Land B.B. 2003. The effect of queen quality on the interactions of workers and dueling queen honey bees (Apis mellifera L.). Behav. Ecol. Sociobiol. 55: 190–196Google Scholar
  16. Hatch S., Tarpy D.R. and Fletcher D.J.C. 1999 Worker regulation of emergency queen rearing in honey bee colonies and the resultant variation in queen quality. Insect. Soc. 46: 372–377Google Scholar
  17. Hirji K.F., Mehta C.R. and Patel N.R. 1987. Computing distributions for exact logistic regression. J. Am. Stat. Assoc. 82: 1110–1117Google Scholar
  18. Jackson J.T., Tarpy D.R. and Fahrbach S.E. 2011. Histological estimates of ovariole number in honey bee queens (Apis mellifera) reveal lack of correlation with other queen-quality measures. J. Insect Sci. 11: 82Google Scholar
  19. Kocher S., Richard F.J., Tarpy D.R. and Grozinger C.M. 2008. Genomic analysis of post-mating changes in the hone bee queen (Apis mellifera). BMC Genomics 9: 232Google Scholar
  20. Kocher S., Richard F.J., Tarpy D.R. and Grozinger C.M. 2009. Queen reproductive state modulates pheromone production and queen-worker interaction sin honey bees. Behav. Ecol. 20: 1007–1014Google Scholar
  21. Koeniger G., Koeniger N., Tingek S. and Phiancharoen M. 2005. Variance in spermatozoa number among Apis dorsata drones and among Apis mellifera drones. Apidologie 36: 279–284Google Scholar
  22. Kraus F.B., Neumann P., Scharpenberg H., van Praagh J. and Moritz R.F.A. 2003. Male fitness of honeybee colonies (A. mellifera L.). J. Evol. Biol. 16: 914–920Google Scholar
  23. Laidlaw H.H. Jr. and Page R. Jr. 1997. Queen Rearing and Bee Breeding. Wicwas, Cheshire, CTGoogle Scholar
  24. Littell R.C., Henry P.R. and Ammerman C.B. 1998. Statistical analysis of repeated measures data using SAS procedures. J. Anim. Sci. 76: 1216–1231Google Scholar
  25. Mehta C.R and Patel N.R. 1995. Exact logistic regression: theory and examples. Stat. Med. 14: 2143–2160Google Scholar
  26. Mitchell C. 1970. Weights of workers and drones. Am. Bee J. 110: 468–469Google Scholar
  27. Nelson D.L. and Gary N.E. 1983. Honey productivity of honey bee Apis mellifera colonies in relation to body weight attractiveness and fecundity of the queen. J. Apic. Res. 22: 209–213Google Scholar
  28. Nelson D.L. and Smirl C. 1977. The effect of queen-related problems and swarming on brood and honey production of honey bee colonies in Manitoba. Manit. Entom. 11: 45–49Google Scholar
  29. Richard F.-J., Schal C., Tarpy D.R. and Grozinger C.M. 2011. Effects of instrumental insemination and insemination quantity on Dufour’s gland chemical profiles and Vitellogenin expression in honey bee queens (Apis mellifera). J. Chem. Ecol. 37: 1027–1036Google Scholar
  30. Richard F.-J., Tarpy D.R. and Grozinger C.M. 2007. Effects of insemination quantity on honey bee queen physiology. PLoS ONE 2: e980Google Scholar
  31. Sanford M.T. 2001. Queen problems. Apis Newsletter (April), University of Florida, GainesvilleGoogle Scholar
  32. Seeley T.D. 1995. The Wisdom of the Hive: The Social Physiology of Honey Bee Colonies. Harvard University Press, Cambridge, MAGoogle Scholar
  33. Seeley T.D. and Visscher P.K. 2003. Choosing a home: how the scouts in a honey bee swarm perceive the completion of their group decision making. Behav. Ecol. Sociobiol. 54: 511–520Google Scholar
  34. Schlüns H., Schlüns E.A., van Praagh J. and Moritz R.F.A. 2003. Sperm numbers in drone honeybees (Apis mellifera) depend on body size. Apidologie 34: 577–584Google Scholar
  35. Sundström L. 1995. Dispersal polymorphism and physiological conditions of males and females in the ant Formica truncorum. Behav. Ecol. 6: 132–139Google Scholar
  36. Tarpy D.R., Hatch S. and Fletcher D.J.C. 2000 The influence of queen age and quality during queen replacement in honeybee colonies. Anim. Behav. 59: 97–101Google Scholar
  37. Tarpy D.R., Gilley D.C. and Seeley T.D. 2004. Levels of selection in a social insect: a review of conflict and cooperation during honey bee (Apis mellifera) queen replacement. Behav. Ecol. Sociobiol. 55: 513–523Google Scholar
  38. Tarpy D.R., Keller J.J., Caren, J.R. and Delaney D.A. 2011. Experimentally induced variation in the physical reproductive potential and mating success in honey bee queens. Insect. Soc. 58: 569–574Google Scholar
  39. Tarpy D.R. and Mayer M.K. 2009. The effects of size and reproductive quality on the outcomes of duels between honey bee queens (Apis mellifera L.). Ethol. Ecol. Evol. 21: 147–153Google Scholar
  40. Toth A.L., Bilof K.B.J, Henshaw M.T., Hunt J.H. and Robinson G.E. 2009. Lipid stores, ovary development, and brain gene expression in Polistes metricus females. Insect. Soc. 56: 77–84Google Scholar
  41. Wallner K. 1999. Varroacides and their residues in bee products. Apidologie 30: 235–248Google Scholar
  42. Webster T.C. 1998. Queen problems - Survey results. Bee Culture. 126: 40–41Google Scholar
  43. Wedmore E.B. 1942. A Manual of Beekeeping. Arnold Press, London, UKGoogle Scholar
  44. Wilson E.O. 1971. The Insect Societies. Harvard University Press, Cambridge, MAGoogle Scholar
  45. Winston M.L. 1987. The Biology of the Honey Bee. Harvard University Press, Cambridge, MAGoogle Scholar
  46. Woyke J. 1971. Correlations between the age at which honeybee brood was grafted, characteristics of the resultant queens, and results of insemination. J. Apic. Res. 10: 45–55Google Scholar

Copyright information

© International Union for the Study of Social Insects (IUSSI) 2012

Authors and Affiliations

  1. 1.Department of EntomologyNorth Carolina State UniversityRaleighUSA
  2. 2.Keck Center for Behavioral BiologyNorth Carolina State UniversityRaleighUSA

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