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Population structure of Apis cerana in Thailand reflects biogeography and current gene flow rather than Varroa mite association

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Abstract

Concordance between the mitochondrial haplotypes of the Eastern honey bee, Apis cerana, and its ectoparasitic Varroa mites across the Isthmus of Kra in Thailand has suggested that local host–pathogen co-evolution may be responsible for the geographic distribution of particular genotypes. To investigate nuclear microsatellites population structure in A. cerana, single workers of A. cerana colonies from Thailand were genotyped at 18 microsatellite loci. The loci showed intermediate to high levels of heterozygosity and a range of allele numbers. The analyses confirmed a fundamental subdivision of the Thai A. cerana population into the “Asia Mainland” and “Sundaland” regions at the Isthmus of Kra. However, the nuclear microsatellite differentiation was less distinct than mtDNA haplotype differences, suggesting male-biased dispersal and population admixture. Overall, samples showed a weak isolation-by-distance effect. The isolated population on Samui island was most differentiated from the other samples. The results do not support our initial hypothesis of local host–pathogen co-evolution, which predicts a strict correspondence between the nuclear genome and the lineage of parasitic Varroa mite of the A. cerana samples, because the gene flow indicated by our nuclear microsatellite markers should also mix potential Varroa resistance alleles among subpopulations. Instead, our study suggests that the coincidental distribution of Varroa lineages and A. cerana population structure in Thailand are the result of biogeographic history and current migration patterns.

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Acknowledgments

We would like to thank Stephen Brown for practical assistance in the lab. Members of the UNCG social insect lab and the North Carolina Honey Bee Research Consortium improved the study through many comments and discussions. The study was further improved by constructive criticisms by three anonymous reviewers and our editor. This study was financially supported by the National Science Foundation (#0615502) and USDA-NIFA (AFRI #2010-65-104-20533).

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Authors and Affiliations

  1. Department of Biology, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA

    O. Rueppell & A. M. Hayes

  2. Department of Biology, Faculty of Sciences, Chulalongkorn University, 10330, Bangkok, Thailand

    N. Warrit

  3. Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, KS, 66045, USA

    D. R. Smith

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  1. O. Rueppell
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  2. A. M. Hayes
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  3. N. Warrit
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  4. D. R. Smith
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Correspondence to O. Rueppell.

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Rueppell, O., Hayes, A.M., Warrit, N. et al. Population structure of Apis cerana in Thailand reflects biogeography and current gene flow rather than Varroa mite association. Insect. Soc. 58, 445–452 (2011). https://doi.org/10.1007/s00040-011-0161-2

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