Abstract
The so far coldest location for flowering plant life was found at 4,505 m asl at the Dom summit in Valais (Switzerland). On an ice-free crest, Saxifraga oppositifolia, a typical arctic-alpine arbuscular mycorrhizal (AM) plant forms several cushions. Here, we report on a surprisingly diverse fungal community of mycorrhizal and non-mycorrhizal species at this particular location. A few grams of fine sand and organic material obtained from the rhizospheric lithic ‘soil’ and fine roots of S. oppositifolia were examined for fungal spores and hyphae. We found five different species of AM fungi (Glomeromycota) that belonged to all three glomeromycotan classes, to four orders and five different families. We also found hyphae of dark-septate endophytes (unidentified on the species and genus level, members of Vibrisseaceae, Helotiales, Ascomycota) and a few cleistothecia of a putative fungal leaf pathogen resembling Podosphaera alpina (Erysiphaceae, Erysiphales, Ascomycota), which is known to cause powdery mildew on S. oppositifolia. Given the size of the cushions and their annual growth, these microcosms of life may not be older than 30 years, obviously sufficient time to establish a rich fungal community despite the extreme cold at this location, which in terms of higher level mycorrhizal fungal taxa is obviously comparable with fungal communities of well-established alpine, mountainous and even temperate lowland grasslands.
References
Błaszkowski J (2012) Glomeromycota. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków
Blumer S (1933) Die Erysiphaceen Mitteleuropas mit besonderer Berücksichtigung der Schweiz. Beiträge zur Kryptogamenflora der Schweiz. 7:1–483
Braun U, Takamatsu S (2000) Phylogeny of Erysiphe, Microsphaera, Uncinula (Erysipheae) and Cystotheca, Podosphaera, Sphaerotheca (Cystotheceae) inferred from rDNA ITS sequences—some taxonomic consequences. Schlechtendalia 4:1–33
Fuchs B, Haselwandter K (2004) Red list plants: colonization by arbuscular mycorrhizal fungi and dark septate endophytes. Mycorrhiza 14:277–281
Giovannetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytol 84:489–500
Grünig CR, Sieber TN (2005) Molecular and phenotypic description of the widespread root symbiont Acephala applanata gen. et sp. nov., formerly known as dark-septate endophyte Type 1. Mycologia 97:628–640
Grünig CR, Queloz V, Duó A, Sieber TN, Holdenrieder O (2008a) Assignment of species rank to six reproductively isolated cryptic species of the Phialocephala fortinii s.l.-Acephala applanata species complex. Mycologia 100:47–67
Grünig CR, Queloz V, Sieber TN, Holdenrieder O (2008b) Dark septate endophytes (DSE) of the Phialocephala fortinii s.l.-Acephala applanata species complex in tree roots: classification, population biology, and ecology. Botany 86:1355–1369
Grünig CR, Queloz V, Duó A, Sieber TN (2009) Phylogeny of Phaeomollisia piceae gen. sp. nov.: a dark, septate, conifer-needle endophyte and its relationships to Phialocephala and Acephala. Mycol Res 113:207–221
Haselwandter K, Read DJ (1980) Fungal associations of roots of dominant and sub-dominant plants in high-alpine vegetation systems with special reference to mycorrhiza. Oecologia 45:57–62
Klimeš L, Doležal J (2010) An experimental assessment of the upper elevational limit of flowering plants in the western Himalayas. Ecography 33:590–596
Körner C (2011) Coldest places on earth with angiosperm plant life. Alp Bot 121:11–22
Körner C (2012) Alpine treelines. Springer, Basel
Mello CMA, Silva GA, Assis DMA, Pontes JS, Ferreira ACA, Leão MPC, Vieira HEE, Maia LC, Oehl F (2013) Paraglomus pernambucanum sp. nov. and Paraglomus bolivianum com. nov., and biogeographic distribution of Paraglomus and Pacispora. J Appl Bot Food Qual 86:113–125
Oehl F, Sieverding E (2004) Pacispora, a new vesicular arbuscular mycorrhizal fungal genus in the Glomeromycetes. J Appl Bot Food Qual 78:72–82
Oehl F, Jansa J, Ineichen K, Mäder P, van der Heijden MGA (2011a) Arbuscular mycorrhizal fungi as bio-indicators in Swiss agricultural soils. Agrarforschung Schweiz 2:304–311
Oehl F, Schneider D, Sieverding E, Burga CA (2011b) Succession of arbuscular mycorrhizal communities in the foreland of the retreating Morteratsch glacier in the Central Alps. Pedobiologia 54:321–331
Oehl F, Sieverding E, Palenzuela J, Ineichen K, Silva GA (2011c) Advances in Glomeromycota taxonomy and classification. IMA Fungus 2:191–199
Oehl F, Silva GA, Goto BT, Maia LC, Sieverding E (2011d) Glomeromycota: two new classes and a new order. Mycotaxon 116:365–379
Oehl F, Palenzuela J, Sánchez-Castro I, Kuss P, Sieverding E, Silva GA (2012) Acaulospora nivalis, a new fungus in the Glomeromycetes, characteristic for high alpine and nival altitudes of the Swiss Alps. Nova Hedwig 95:105–122
Palenzuela J, Azcón-Aguilar C, Barea JM, Silva GA, Oehl F (2013) Acaulospora pustulata and Acaulospora tortuosa, two new species in the Glomeromycota from Sierra Nevada (southern Spain). Nova Hedwig 97:305–319
Queloz V, Sieber TN, Holderrieder O, McDonald BA, Grünig RR (2011) No biogeographical pattern for a root-associated fungal species complex. Glob Ecol Biogeogr 20:160–169
Sieverding E (1991) Vesicular-arbuscular mycorrhiza management in tropical agrosystems. Deutsche Gesellschaft für Technische Zusammenarbeit Nr. 224. Hartmut Bremer Verlag, Friedland, Germany
Smith SE, Read DA (2008) Mycorrhizal Symbiosis. 3rd edition. Academic Pres Ltd., Cambridge, UK, p. 787
Spain JL (2003) Emendation of Archaeospora and of its type species, Archaeospora trappei. Mycotaxon 87:109–112
Wetzel K, Silva GA, Matczinski U, Oehl F, Fester T (2014) Superior differentiation of arbuscular mycorrhizal fungal communities from till and no-till plots by morphological spore identification when compared to T-RFLP. Soil Biol Biochem 72:88–96
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Oehl, F., Körner, C. Multiple mycorrhization at the coldest place known for Angiosperm plant life. Alp Botany 124, 193–198 (2014). https://doi.org/10.1007/s00035-014-0138-7
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DOI: https://doi.org/10.1007/s00035-014-0138-7