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A common ancestor for a subunit in the mitochondrial proton-translocating NADH:ubiquinone oxidoreductase (complex I) and short-chain dehydrogenases/reductases

Abstract.

The proton-translocating NADH:ubiquinone oxidoreductase or complex I is located in the inner membranes of mitochondria, where it catalyzes the transfer of electrons from NADH to ubiquinone. Here we report that one of the subunits in complex I is homologous to short-chain dehydrogenases and reductases, a family of enzymes with diverse activities that include metabolizing steroids, prostaglandins and nucleotide sugars. We discovered that a subunit of complex I in human, cow, Neurospora crassa and Aquifex aeolius is homologous to nucleotide-sugar epimerases and hydroxysteroid dehydrogenases while seeking distant homologs of these enzymes with a hidden Markov model-based search of Genpept. This homology allows us to use information from the solved three-dimensional structures of nucleotide-sugar epimerases and hydroxysteroid dehydrogenases and our motif analysis of these enzymes to predict functional domains on their homologs in complex I.

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Received 26 November 1998; received after revision 12 January 1999; accepted 12 January 1999

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Baker, M., Grundy, W. & Elkan, C. A common ancestor for a subunit in the mitochondrial proton-translocating NADH:ubiquinone oxidoreductase (complex I) and short-chain dehydrogenases/reductases. CMLS, Cell. Mol. Life Sci. 55, 450–455 (1999). https://doi.org/10.1007/s000180050301

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  • DOI: https://doi.org/10.1007/s000180050301

  • Key words. NADH:ubiquinone oxidoreductase; short-chain dehydrogenases/reductases; nucleotide-sugar epimerases; hydroxysteroid dehydrogenases; evolution.