Abstract
Surgical crushing of stones alone has not addressed the increasing prevalence of kidney stones. A promising strategy is to tackle the kidney damage and crystal aggregation inherent in kidney stones with the appropriate therapeutic target. FKBP prolyl isomerase 5 (FKBP5) is a potential predictor of kidney injury, but its status in calcium oxalate (CaOx) kidney stones is not clear. This study attempted to elucidate the role and mechanism of FKBP5 in CaOx kidney stones. Lentivirus and adeno-associated virus were used to control FKBP5 expression in a CaOx kidney stone model. Transcriptomic sequencing and immunological assays were used to analyze the mechanism of FKBP5 deficiency in CaOx kidney stones. The results showed that FKBP5 deficiency reduced renal tubular epithelial cells (RTEC) apoptosis and promoted cell proliferation by downregulating BOK expression. It also attenuated cell–crystal adhesion by downregulating the expression of CDH4. In addition, it inhibited M1 polarization and chemotaxis of macrophages by suppressing CXCL10 expression in RTEC. Moreover, the above therapeutic effects were exerted by inhibiting the activation of NF-κB signaling. Finally, in vivo experiments showed that FKBP5 deficiency attenuated stone aggregation and kidney injury in mice. In conclusion, this study reveals that FKBP5 deficiency attenuates cell–crystal adhesion, reduces apoptosis, promotes cell proliferation, and inhibits macrophage M1 polarization and chemotaxis by inhibiting NF-κB signaling. This provides a potential therapeutic target for CaOx kidney stones.
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The raw data of RNA-seq were shown in Supplementary Information 2. Other data will be made available on request.
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This work was supported by the National Natural Science Foundation of China (No. 82070723) and (No. 82270797).
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SXY, LJX, and QLS designed the research and wrote the manuscript; QLS, CS, XC, LJL, and WBL performed the experiments; CS, WBL, and LJX analyzed data; QLS, XC, CS, and YHX performed the animal model, and performed critical reading/editing of the manuscript; SXY and LJX supervised the study.
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Song, Q., Song, C., Chen, X. et al. FKBP5 deficiency attenuates calcium oxalate kidney stone formation by suppressing cell–crystal adhesion, apoptosis and macrophage M1 polarization via inhibition of NF-κB signaling. Cell. Mol. Life Sci. 80, 301 (2023). https://doi.org/10.1007/s00018-023-04958-7
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DOI: https://doi.org/10.1007/s00018-023-04958-7