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Autophagosome–lysosome fusion is facilitated by plectin-stabilized actin and keratin 8 during macroautophagic process

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Abstract

Autophagy is a lysosome-mediated degradative process that removes damaged proteins and organelles, during which autophagosome–lysosome fusion is a key step of the autophagic flux. Based on our observation that intermediate cytofilament keratin 8 (KRT8) enhances autophagic clearance in cells under oxidative stress condition, we investigated whether KRT8 supports the cytoplasmic architectural networks to facilitate the vesicular fusion entailing trafficking onto filamentous tracks. We found that KRT8 interacts with actin filaments via the cytolinker, plectin (PLEC) during trafficking of autophagosome. When PLEC was knocked down or KRT8 structure was collapsed by phosphorylation, autophagosome–lysosome fusion was attenuated. Inhibition of actin polymerization resulted in accumulation of autophagosomes owing to a decrease in autophagosome and lysosome fusion. Furthermore, myosin motor protein was found to be responsible for vesicular trafficking along the actin filaments to entail autolysosome formation. Thus, the autophagosome–lysosome fusion is aided by PLEC-stabilized actin filaments as well as intermediate cytofilament KRT8 that supports the structural integrity of actin filaments during macroautophagic process under oxidative stress condition.

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Funding

This research was supported by the grant from the National Research Foundation of Korea (2017R1E1A1A01074656) funded by the Korean government.

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  1. Sumin Son and Ahruem Baek contributed equally to this work.

Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea

    Sumin Son & Dong-Eun Kim

  2. Safety Measurement Institute, Korea Research Institute of Standards and Science, 267 Gajeong-Ro, Yuseong-Gu, Daejeon, 34113, Republic of Korea

    Ahruem Baek

  3. Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam, 13120, Republic of Korea

    Jong Hun Lee

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D-EK and AB formulated the hypothesis, and initiated and organized the study. SS and AB designed and performed experiments with data analysis. SS, AB, JHL and D-EK wrote the manuscript.

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Correspondence to Dong-Eun Kim.

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Son, S., Baek, A., Lee, J.H. et al. Autophagosome–lysosome fusion is facilitated by plectin-stabilized actin and keratin 8 during macroautophagic process. Cell. Mol. Life Sci. 79, 95 (2022). https://doi.org/10.1007/s00018-022-04144-1

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