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Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis

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Abstract

Availability of iron is a key factor in the survival and multiplication of Mycobacterium tuberculosis (M.tb) within host macrophage phagosomes. Despite host cell iron regulatory machineries attempts to deny supply of this essential micronutrient, intraphagosomal M.tb continues to access extracellular iron. In the current study, we report that intracellular M.tb exploits mammalian secreted Glyceraldehyde 3-phosphate dehydrogenase (sGAPDH) for the delivery of host iron carrier proteins lactoferrin (Lf) and transferrin (Tf). Studying the trafficking of iron carriers in infected cells we observed that sGAPDH along with the iron carrier proteins are preferentially internalized into infected cells and trafficked to M.tb containing phagosomes where they are internalized by resident mycobacteria resulting in iron delivery. Collectively our findings provide a new mechanism of iron acquisition by M.tb involving the hijack of host sGAPDH. This may contribute to its successful pathogenesis and provide an option for targeted therapeutic intervention.

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Abbreviations

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

Tf:

Transferrin

Lf:

Lactoferrin

TfR:

Transferrin receptor

SFM:

Serum free medium

M.tb :

Mycobacterium tuberculosis

LAM:

Lipoarabinomannan

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Acknowledgements

Mr. Anil Theophilus & Mr. Randeep Sharma are acknowledged for technical assistance. This is IMTECH communication No. 035/2021.

Funding

S.C. and R.S.M. received fellowships from University Grants Commission India, A.D., A. P., G.K.C. and S.T. from Department of Biotechnology, India and R.D. was supported by a fellowship of the Indian Council of Medical Research. Partial Financial support was received from CSIR, DBT (Project no's. BT/PR13469/BRB/10/1395/2015 and BT/PR14292/NNT/28/853/2015), ICMR and DST (Project no. EMR/2016/001898).

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  1. Anil Patidar and Himanshu Malhotra contributed equally.

Authors and Affiliations

  1. Institute of Microbial Technology, CSIR, Sector 39A, Chandigarh, 160036, India

    Anil Patidar, Himanshu Malhotra, Surbhi Chaudhary, Manoj Kumar, Rahul Dilawari, Gaurav Kumar Chaubey, Asmita Dhiman, Radheshyam Modanwal, Sharmila Talukdar & Manoj Raje

  2. National Institute of Pharmaceutical Education and Research, Phase X, Sector 67, SAS Nagar, Punjab, 160062, India

    Chaaya Iyengar Raje

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  1. Anil Patidar
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Contributions

CIR and MR conceptualized and planned the research work. AP, HM AD, SC, RD, GKC, ST and RSM all carried out the experiments in the manuscript and compiled the preliminary data, AP, HM, CIR and MR analyzed the data and compiled the manuscript. All authors read and approved the final manuscript.

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Correspondence to Manoj Raje.

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Patidar, A., Malhotra, H., Chaudhary, S. et al. Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis. Cell. Mol. Life Sci. 79, 62 (2022). https://doi.org/10.1007/s00018-021-04110-3

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