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Hedgehog signaling regulates regenerative patterning and growth in Harmonia axyridis leg

Cellular and Molecular Life Sciences volume 78, pages 2185–2197 (2021)Cite this article

Abstract

Appendage regeneration has been widely studied in many species. Compared to other animal models, Harmonia axyridis has the advantage of a short life cycle, is easily reared, has strong regeneration capacity and contains systemic RNAi, making it a model organism for research on appendage regeneration. Here, we performed transcriptome analysis, followed by gene functional assays to reveal the molecular mechanism of H. axyridis leg regenerative growth process. Signaling pathways including Decapentaplegic (Dpp), Wingless (Wg), Ds/Ft/Hippo, Notch, Egfr, and Hedgehog (Hh) were all upregulated during the leg regenerative patterning and growth. Among these, Hh and its auxiliary receptor Lrp2 were required for the proper patterning and growth of the regenerative leg. The targets of canonical Hh signaling were required for the regenerative growth which contributes to the leg length, but were not essential for the pattern formation of the regenerative leg. dpp, wg and leg developmental-related genes including rn, dac and Dll were all regulated by hh and lrp2 and may play an essential role in the regenerative patterning of the leg.

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Acknowledgement

This research was financially supported by BJNSF6182020, NSFC31872293 and 31872295.

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  1. Department of Entomology and MOA Lab for Pest Monitoring and Green Management, China Agricultural University, Beijing, 100193, China

    Hang Zhou, Zhongzheng Ma, Zhiqi Wang, Shuo Yan, Dan Wang & Jie Shen

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  1. Hang Zhou
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  2. Zhongzheng Ma
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HZ, ZM, and ZW performed experiments. HZ, JS, SY, and DW designed the experiments, analyzed and interpreted the data, and wrote the manuscript.

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Correspondence to Dan Wang or Jie Shen.

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Zhou, H., Ma, Z., Wang, Z. et al. Hedgehog signaling regulates regenerative patterning and growth in Harmonia axyridis leg. Cell. Mol. Life Sci. 78, 2185–2197 (2021). https://doi.org/10.1007/s00018-020-03631-7

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Keywords

  • Ladybug
  • Leg amputation
  • Leg regeneration
  • Appendage regeneration
  • Blastema