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Multiple roles of CTDK-I throughout the cell

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Abstract

The heterotrimeric carboxy-terminal domain kinase I (CTDK-I) in yeast is a cyclin-dependent kinase complex that is evolutionally conserved throughout eukaryotes and phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II (RNApII) on the second-position serine (Ser2) residue of YSPTSPS heptapeptide repeats. CTDK-I plays indispensable roles in transcription elongation and transcription-coupled processing, such as the 3′-end processing of nascent mRNA transcripts. However, recent studies have revealed additional roles of CTDK-I beyond its primary effect on transcription by RNApII. Here, we describe recent advances in the regulation of genomic stability and rDNA integrity by CTDK-I and highlight the previously underappreciated cellular roles of CTDK-I in rRNA synthesis by RNA polymerase I and translational initiation and elongation. These multiple roles of CTDK-I throughout the cell expand our understanding of how this complex functions to coordinate diverse cellular processes through gene expression and how the human orthologue exerts its roles in diseased states such as tumorigenesis.

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Acknowledgements

We are grateful to Stephen Buratowski for suggestions and insightful comments on the manuscript. This work was supported by a National Research Foundation of Korea (NRF) Grant funded by the South Korean government (no. NRF-2016R1A2B2008217).

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  1. Rakesh Srivastava and Ruxin Duan contributed equally to this work.

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  1. Plant Molecular Biology and Genetic Engineering Division, CSIR-National Botanical Research Institute, Lucknow, U.P., 226001, India

    Rakesh Srivastava

  2. Department of Molecular and Life Science, College of Science and Convergence Technology, Hanyang University ERICA Campus, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Republic of Korea

    Ruxin Duan & Seong Hoon Ahn

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Srivastava, R., Duan, R. & Ahn, S.H. Multiple roles of CTDK-I throughout the cell. Cell. Mol. Life Sci. 76, 2789–2797 (2019). https://doi.org/10.1007/s00018-019-03118-0

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