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The equilibrium of ubiquitination and deubiquitination at PLK1 regulates sister chromatid separation

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Abstract

PLK1 regulates almost every aspect of mitotic events, including mitotic entry, spindle assembly, chromosome alignment, sister chromatid segregation, metaphase-anaphase transition, cytokinesis, etc. In regulating the chromosome alignment and sister chromatid segregation, PLK1 has to be localized to and removed from kinetochores at the right times, and the underlying mechanism that regulates PLK1 both spatially and temporally only became clearer recently. It has been found that deubiquitination and ubiquitination of PLK1 are responsible for its localization to and dissociation from the kinetochores, respectively. The equilibrium of this ubiquitination and deubiquitination plays an important role in regulating proper chromosome alignment and timely sister chromatid segregation. Here, we summarize and discuss the recent findings in investigating the spatial and temporal regulation of PLK1 during chromosome alignment and sister chromatid segregation.

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Abbreviations

PLK1:

Polo-like kinase 1

Usp16:

Ubiquitin-specific peptidase 16

CUL3:

Cullin 3

SAC:

Spindle assembly checkpoint

KT–MT:

Kinetochore–microtubule

PB:

Polo box

PBD:

Polo box domain

PBIP1:

Polo box domain-interacting protein 1

INCENP:

Inner centromere protein

APC/C:

Anaphase-promoting complex/cyclosome

CCAN:

Constitutive centromere-associated network

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Acknowledgements

We thank other members of our laboratories for helpful comments and critical discussion on this work. Work in Liu laboratory is supported by a NIH Grant (5SC2GM089622-03), and in Zhang laboratory by the National Natural Science Foundation of China (NSFC) (31520103906, 31371365, and 31430051) and the Ministry of Science and Technology of China (2016YFA0100501 and 2016YFA0500201).

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  1. Department of Biological Sciences, California State Polytechnic University, Pomona, CA, 91768, USA

    Junjun Liu

  2. The Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing, 100871, China

    Chuanmao Zhang

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  1. Junjun Liu
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Correspondence to Junjun Liu or Chuanmao Zhang.

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Liu, J., Zhang, C. The equilibrium of ubiquitination and deubiquitination at PLK1 regulates sister chromatid separation. Cell. Mol. Life Sci. 74, 2127–2134 (2017). https://doi.org/10.1007/s00018-017-2457-5

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