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IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells

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Abstract

Aurora B activation is triggered at the mitotic entry and required for proper microtubule-kinetochore attachment at mitotic phase. Therefore, Aurora B should be in inactive form in interphase to prevent aberrant cell cycle progression. However, it is unclear how the inactivation of Aurora B is sustained during interphase. In this study, we find that IK depletion-induced mitotic arrest leads to G2 arrest by Aurora B inhibition, indicating that IK depletion enhances Aurora B activation before mitotic entry. IK binds to Aurora B, and colocalizes on the nuclear foci during interphase. Our data further show that IK inhibits Aurora B activation through recruiting PP2A into IK and Aurora B complex. It is thus believed that IK, as a scaffold protein, guides PP2A into Aurora B to suppress its activity in interphase until mitotic entry.

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Abbreviations

IK:

Inhibitor K562

PP2A:

Protein phosphatase 2A

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Acknowledgments

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2015R1D1A4A01016293) and Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (2015016662) and (No. 2011-0030074).

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Authors and Affiliations

  1. Division of Biological Sciences, Department of Life Systems, Research Center for Women’s Disease, Sookmyung Women’s University, Seoul, 140-742, Republic of Korea

    Sunyi Lee, Ae Lee Jeong, Jeong Su Park, Sora Han, Keun Il Kim, Yonghwan Kim, Jong Hoon Park, Jong-Seok Lim, Myung Sok Lee & Young Yang

  2. Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women’s University, Seoul, 140-742, Republic of Korea

    Chang-Young Jang

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  1. Sunyi Lee
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Lee, S., Jeong, A.L., Park, J.S. et al. IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells. Cell. Mol. Life Sci. 73, 3375–3386 (2016). https://doi.org/10.1007/s00018-016-2162-9

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