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Mechanism, factors, and physiological role of nonsense-mediated mRNA decay

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Abstract

Nonsense-mediated mRNA decay (NMD) is a translation-dependent, multistep process that degrades irregular or faulty messenger RNAs (mRNAs). NMD mainly targets mRNAs with a truncated open reading frame (ORF) due to premature termination codons (PTCs). In addition, NMD also regulates the expression of different types of endogenous mRNA substrates. A multitude of factors are involved in the tight regulation of the NMD mechanism. In this review, we focus on the molecular mechanism of mammalian NMD. Based on the published data, we discuss the involvement of translation termination in NMD initiation. Furthermore, we provide a detailed overview of the core NMD machinery, as well as several peripheral NMD factors, and discuss their function. Finally, we present an overview of diseases associated with NMD factor mutations and summarize the current state of treatment for genetic disorders caused by nonsense mutations.

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Acknowledgments

This research was funded by grants from the Fritz Thyssen Stiftung and the Deutsche Forschungsgemeinschaft (SFB635, project B06) to N.H.G.

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    1. Institute for Genetics, University of Cologne, Cologne, Germany

      Tobias Fatscher, Volker Boehm & Niels H. Gehring

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    Correspondence to Niels H. Gehring.

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    T. Fatscher and V. Boehm contributed equally to this work.

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    Fatscher, T., Boehm, V. & Gehring, N.H. Mechanism, factors, and physiological role of nonsense-mediated mRNA decay. Cell. Mol. Life Sci. 72, 4523–4544 (2015). https://doi.org/10.1007/s00018-015-2017-9

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