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Quality control of homologous recombination

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Abstract

Exogenous and endogenous genotoxic agents, such as ionizing radiation and numerous chemical agents, cause DNA double-strand breaks (DSBs), which are highly toxic and lead to genomic instability or tumorigenesis if not repaired accurately and efficiently. Cells have over evolutionary time developed certain repair mechanisms in response to DSBs to maintain genomic integrity. Major DSB repair mechanisms include non-homologous end joining and homologous recombination (HR). Using sister homologues as templates, HR is a high-fidelity repair pathway that can rejoin DSBs without introducing mutations. However, HR execution without appropriate guarding may lead to more severe gross genome rearrangements. Here we review current knowledge regarding the factors and mechanisms required for accomplishment of accurate HR.

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Acknowledgments

We apologize to colleagues whose work could not be cited due to space limitations. We would like to thank all our colleagues in the Huang laboratory for insightful discussions. This work was supported in part by National Program for Special Support of Eminent Professionals, National Basic Research Program of China Grants 2012CB944402 and 2013CB911003, National Natural Science Funds for Distinguished Young Scholar, National Natural Science Foundation of China Grant 31071243, Zhejiang University K.P. Chao’s High Technology Development Foundation, and the China’s Fundamental Research Funds for the Central Universities. Ting Liu is a member of Feng lab and supported by National Natural Science Foundation of China grant (31171347 and 31090360) and MOST Grants 2012CB966600 and 2013CB945303.

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  1. Life Sciences Institute, Zhejiang University, Hangzhou, 310058, Zhejiang, China

    Ting Liu & Jun Huang

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Liu, T., Huang, J. Quality control of homologous recombination. Cell. Mol. Life Sci. 71, 3779–3797 (2014). https://doi.org/10.1007/s00018-014-1649-5

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