Abstract
Matricellular proteins interact with the extracellular matrix (ECM) and modulate cellular processes by binding to cell surface receptors and initiating intracellular signal transduction. Their association with the ECM and the ability of some members of this protein family to regulate cell motility have opened up new avenues of research to investigate their functions in normal and diseased cells. In this review, we summarize the research on CyrA, an ECM calmodulin-binding protein in Dictyostelium. CyrA is proteolytically cleaved into smaller EGF-like (EGFL) repeat containing cleavage products during development. The first EGFL repeat of CyrA binds to the cell surface and activates a novel signalling pathway that modulates cell motility in this model organism. The similarity of CyrA to the most well-characterized matricellular proteins in mammals allows it to be designated as the first matricellular protein identified in Dictyostelium.
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Abbreviations
- AcbA:
-
Acyl-CoA binding protein A
- ADAMTS:
-
A disintegrin and mettaloproteinase with thrombospondin motifs
- carA:
-
cAMP receptor A
- carC:
-
cAMP receptor C
- CaM:
-
Calmodulin
- CaMBP:
-
CaM-binding protein
- CyrA:
-
Cysteine-rich protein A
- CyrA-C40:
-
40 kDa CyrA cleavage product
- CyrA-C45:
-
45 kDa CyrA cleavage product
- ECM:
-
Extracellular matrix
- EGFL:
-
Epidermal growth factor-like
- EGFL1:
-
CyrA EGFL repeat 1
- EGFR:
-
EGF receptor
- ER:
-
Endoplasmic reticulum
- MHC:
-
Myosin II heavy chain
- MMP2:
-
Matrix metalloproteinase 2
- PaxB:
-
Paxillin B
- PI3K:
-
Phosphatidylinositol-3-kinase
- PKA:
-
Protein kinase A
- PLA2:
-
Phospholipase A2
- PLC:
-
Phospholipase C
- SDF-2:
-
Spore differentiation factor 2
- SPARC:
-
Secreted protein acidic and rich in cysteine
- TalB:
-
Talin B
- Ten14:
-
14th EGFL repeat of tenascin C
- Tenascin C:
-
Tenascin cytotactin
- TSP:
-
Thrombospondin
- VinB:
-
Vinculin B
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Acknowledgments
This review was supported by a Discovery Grant (D.H.O’D.; A6807) and a Canada Graduate Scholarship (R.J.H.) from the Natural Sciences and Engineering Research Council of Canada.
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Huber, R.J., O’Day, D.H. A matricellular protein and EGF-like repeat signalling in the social amoebozoan Dictyostelium discoideum . Cell. Mol. Life Sci. 69, 3989–3997 (2012). https://doi.org/10.1007/s00018-012-1068-4
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DOI: https://doi.org/10.1007/s00018-012-1068-4