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E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly

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Abstract

A decrease in the levels of the desmosomal plaque protein, plakophilin3 (PKP3), leads to a decrease in desmosome size and cell-cell adhesion. To test the hypothesis that PKP3 is required for desmosome formation, the recruitment of desmosomal components to the cell surface was studied in the PKP3 knockdown clones. The PKP3 knockdown clones showed decreased cell border staining for multiple desmosomal proteins, when compared to vector controls, and did not form desmosomes in a calcium switch assay. Further analysis demonstrated that PKP3, plakoglobin (PG) and E-cadherin are present at the cell border at low concentrations of calcium. Loss of either PG or E-cadherin led to a decrease in the levels of PKP3 and other desmosomal proteins at the cell border. The results reported here are consistent with the model that PG and E-cadherin recruit PKP3 to the cell border to initiate desmosome formation.

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Abbreviations

ARM:

Armadillo

WT:

Wild type

PKP:

Plakophilin

PG:

Plakoglobin

DP:

Desmoplakin

DSG:

Desmoglein

DSC:

Desmocollin

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Acknowledgements

We thank the ACTREC confocal and electron microscope facilities and Dr. S. Shirolikar and Mr. L. Borde at the TIFR electron microscope facility for help with acquiring the confocal and electron microscope images, respectively, and M. Sonawane from TIFR for antibodies to E-cadherin, ZO-1 and α-catenin. S.T.K. and P.G. are supported by fellowships from the University Grants Commission. This work was supported in part by grants from the Department of Science and Technology (DST) and ACTREC.

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Authors and Affiliations

  1. KS215, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar Node, Navi Mumbai, 410210, India

    Prajakta Gosavi, Samrat T. Kundu, Nileema Khapare, Lalit Sehgal, Mansi S. Karkhanis & Sorab N. Dalal

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  1. Prajakta Gosavi
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  2. Samrat T. Kundu
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  3. Nileema Khapare
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  6. Sorab N. Dalal
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Correspondence to Sorab N. Dalal.

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Prajakta Gosavi and Samrat T. Kundu contributed equally to the work.

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Gosavi, P., Kundu, S.T., Khapare, N. et al. E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly. Cell. Mol. Life Sci. 68, 1439–1454 (2011). https://doi.org/10.1007/s00018-010-0531-3

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