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Involvement of aryl hydrocarbon receptor nuclear translocator in EGF-induced c-Jun/Sp1-mediated gene expression

Abstract

Aryl hydrocarbon receptor nuclear translocator (ARNT) binds to other basic helix-loop-helix Per/ARNT/Sim (bHLH-PAS) proteins to form functional transcriptional complexes in order to regulate specific biological pathways. Here, we report a novel mechanism that upon EGF treatment, ARNT associated with non-bHLH-PAS transcription factors, c-Jun/Sp1, and regulated gene expression, through forming a c-Jun/ARNT/Sp1 complex and binding to the Sp1 site of the gene promoter. EGF-induced promoter activity and the mRNA level of 12(S)-lipoxygenase as well as the association between c-Jun and Sp1 were reduced by ARNT knockdown. Notably, dominant negative c-Jun mutant, TAM-67, blocked ARNT-mediated 12(S)-lipoxygenase expression, demonstrating that c-Jun was responsible for the transcriptional activation. Moreover, ARNT knockdown also inhibited other EGF-induced c-Jun/Sp1 mediated gene expression, such as p21WAF1/CIP1. Our results reveal a novel mechanism by which ARNT acts as a modulator to bridge the c-Jun/Sp1 interaction and plays a role in EGF-mediated gene expression under normoxic conditions.

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Acknowledgments

This work was supported by grant NSC 98-2320-B-006-014 from the National Science Council of the Republic of China and by the National Cheng Kung University Project of Promoting Academic Excellence and Developing World-Class Research Centers.

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Correspondence to Wen-Chang Chang or Ben-Kuen Chen.

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W.-C. Huang and S.-T. Chen contributed equally to this work.

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Huang, WC., Chen, ST., Chang, WC. et al. Involvement of aryl hydrocarbon receptor nuclear translocator in EGF-induced c-Jun/Sp1-mediated gene expression. Cell. Mol. Life Sci. 67, 3523–3533 (2010). https://doi.org/10.1007/s00018-010-0392-9

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  • DOI: https://doi.org/10.1007/s00018-010-0392-9

Keywords

  • Epidermal growth factor (EGF)
  • Aryl hydrocarbon receptor nuclear translocator (ARNT)
  • Gene expression
  • c-Jun/Sp1
  • Protein–DNA interaction