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Acetylcholinesterase in intestinal cell differentiation involves G2/M cell cycle arrest

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Abstract.

Here we examine differentiation of the intestinal cell line Caco-2 following exposure to sodium butyrate (NaBT), using alkaline phosphatase (ALP) activity and carcinoembryonic antigen (CEA) levels as markers of differentiation. We show that acetylcholinesterase (AChE) activity and RNA levels increase during differentiation. Treatment with AChE inhibitors or knockdown of AChE levels by shRNA markedly decrease ALP and CEA levels in a concentration- and time-dependent manner. Finally, our observations suggest that NaBT-induced differentiation of intestinal cells involves AChE-induced cell cycle arrest.

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Authors and Affiliations

  1. Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, 320 YueYang Road, Shanghai, 200031, China

    A. C. Xiang, J. Xie & X. J. Zhang

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  1. A. C. Xiang
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  2. J. Xie
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  3. X. J. Zhang
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Correspondence to X. J. Zhang.

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Received 11 January 2008; received after revision 9 April 2008; accepted 11 April 2008

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Xiang, A.C., Xie, J. & Zhang, X.J. Acetylcholinesterase in intestinal cell differentiation involves G2/M cell cycle arrest. Cell. Mol. Life Sci. 65, 1768–1779 (2008). https://doi.org/10.1007/s00018-008-8016-3

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  • DOI: https://doi.org/10.1007/s00018-008-8016-3

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