Abstract.
Transcription factor C/EBPs are involved in the regulation of various cellular responses. Here, it was suggested that C/EBPδ gene was activated by lipopolysaccharide (LPS) through transcription factors Sp1, c-Rel, and c-Jun. Assay of the luciferase reporter vectors containing a 5′-deletion of the C/EBPδ gene promoter indicated that a LPS-responsive element was positioned between –345 and –35 bp of mouse C/EBPδ gene promoter. Transcription factors Sp1, c-Rel, and c-Jun bound to this region were identified using both in vivo chromatin immunoprecipitation and in vitro DNA-protein binding assays. LPS enhanced the proteins and DNA binding capacities of c-Rel and c-Jun, and the downstream Sp1 site was essential for LPS-induced C/EBPδ gene. Treatment of cells with ERK/JNK/p38 inhibitors or NF-κB inhibitor inhibited the LPS-induced C/EBPδ gene expression by inhibiting c-Jun, c-Rel, and p300 binding to DNA. Our findings provide a better understanding of LPS-induced C/EBPδ gene expression.
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Received 14 August 2007; received after revision 6 October 2007; accepted 10 October 2007
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Liu, YW., Chen, CC., Wang, JM. et al. Role of transcriptional factors Sp1, c-Rel, and c-Jun in LPS-induced C/EBPδ gene expression of mouse macrophages. Cell. Mol. Life Sci. 64, 3282–3294 (2007). https://doi.org/10.1007/s00018-007-7375-5
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DOI: https://doi.org/10.1007/s00018-007-7375-5