Cellular and Molecular Life Sciences

, Volume 64, Issue 3, pp 365–376

Identification of steroid hormone signaling pathway in insect cell differentiation

  • D. Siaussat
  • F. Bozzolan
  • P. Porcheron
  • S. Debernard
Research Article

Abstract.

To dissect the steroid hormone signaling pathway involved in insect cell morphological differentiation, we extended the application of the doublestranded RNA-mediated interference (dsRNAi) method to the epidermal IAL-PID2 cell line from Plodia interpunctella Lepidoptera. We first demonstrated that dsRNA was capable of efficiently blocking the steroid hormone 20-hydroxyecdysone (20E) inducibility of proteins that belong to the nuclear receptor superfamily, including the ecdysone receptor (EcR), its partner Ultraspiracle (USP), the insect homolog of the vertebrate retinoid X receptor and the HR3 transcription factor. We then showed that inhibiting the 20E induction of EcR, USP or HR3 proteins prevented the increased synthesis of β tubulin and consequently the morphological transformation of cells. Thanks to this functional approach, we have shown, for the first time, the participation of EcR, USP and HR3 in a 20E signaling pathway that directs morphological differentiation in insect cells by regulating β tubulin expression.

Keywords.

Steroid hormone receptor superfamily cell morphological differentiation RNA interference β tubulin subnit signaling pathway 

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Copyright information

© Birkhäuser Verlag, Basel 2007

Authors and Affiliations

  • D. Siaussat
    • 1
  • F. Bozzolan
    • 1
  • P. Porcheron
    • 1
  • S. Debernard
    • 1
  1. 1.UMR 1272 Physiologie de l’Insecte, Signalisation et CommunicationUniversité Pierre et Marie CurieParisFrance

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