Abstract.
Dendritic cells (DC) are specialized antigen-presenting cells. Bone marrow monocytes have been widely used to generate murine myeloid DC. We found that mouse macrophages derived from bone marrow CD11b+ monocytes influenced the differentiation of these precursors into DC. Modulation of differentiation was demonstrated by the down-regulation of CD11c, CD40, and CD86 expression and by IL-12 production. DC differentiated in the presence of conditioned medium from bone marrow-derived macrophage culture (MCM) had impaired ability to stimulate proliferation of, and IFN- γ production by, allogeneic CD4+ T cells. This inhibition of DC differentiation was mainly mediated by secretory products from macrophages but not by cell-cell contact. MCM contained higher concentrations of macrophage-colony-stimulating factor (M-CSF), IL-10, and TGF- β1, whereas IL-6 remained unchanged compared with conditioned medium from fresh monocytes. M-CSF may be the major mediator in MCM inhibiting DC differentiation. This study demonstrates an important influence of bone marrow-derived macrophages on DC precursors during DC differentiation.
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Received 12 September 2006; received after revision 20 October 2006; accepted 13 November 2006
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Liao, H.F., Yang, Y.C., Chen, Y.Y. et al. Macrophages derived from bone marrow modulate differentiation of myeloid dendritic cells. Cell. Mol. Life Sci. 64, 104–111 (2007). https://doi.org/10.1007/s00018-006-6407-x
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DOI: https://doi.org/10.1007/s00018-006-6407-x