Abstract
The three-dimensional, atomic-resolution protein structures produced by X-ray crystallography over the past 50+ years have led to tremendous chemical understanding of fundamental biochemical processes. The pace of discovery in protein crystallography has increased greatly with advances in molecular biology, crystallization techniques, cryocrystallography, area detectors, synchrotrons and computing. While the methods used to produce single, well-ordered crystals have also evolved over the years in response to increased understanding and advancing technology, crystallization strategies continue to be rooted in trial-and-error approaches. This review summarizes the current approaches in protein crystallization and surveys the first results to emerge from the structural genomics efforts.
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Received 1 July 2003; received after revision 6 August 2003; accepted 22 August 2003
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Kundrot, C.E. Which strategy for a protein crystallization project?. CMLS, Cell. Mol. Life Sci. 61, 525–536 (2004). https://doi.org/10.1007/s00018-003-3260-z
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DOI: https://doi.org/10.1007/s00018-003-3260-z