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Multidrug-resistance-associated protein MGr1-Ag is identical to the human 37-kDa laminin receptor precursor

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We report the isolation and functional characterization of the gene encoding MGr1-Ag, a multidrug-resistance-associated protein. A λgt11 cDNA library derived from colorectal carcinoma SW480 cells was screened with monoclonal antibody MGr1. DNA homology analysis of 22 positive clones (designated R1–R22) suggested human 37-kDa laminin receptor precursor (37LRP, R7/R9/R15/R16/R19/R20) and a novel gene (R22) as candidate genes encoding MGr1-Ag. Western blot analysis showed that anti-R20 serum reacted with a unique protein band that was consistent with MGr1-Ag, while anti-R22 serum could not react with MGr1-Ag. The coding gene for MGr1-Ag was amplified using reverse transcription-PCR. Sequence analysis revealed that the MGr1-Ag and 37LRP genes shared the same coding sequence. An in vitro drug sensitivity assay indicated that down-regulation of 37LRP by an antisense technique could significantly enhance the cytotoxicity of anticancer drugs to gastric cancer cells. Thus we draw the conclusion that MGr1-Ag is identical to 37LRP.

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Received 1 June 2002; received after revision 1 July 2002; accepted 10 July 2002

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Shi, Y., Zhai, H., Wang, X. et al. Multidrug-resistance-associated protein MGr1-Ag is identical to the human 37-kDa laminin receptor precursor. CMLS, Cell. Mol. Life Sci. 59, 1577–1583 (2002). https://doi.org/10.1007/s00018-002-8531-6

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  • DOI: https://doi.org/10.1007/s00018-002-8531-6

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