Abstract.
The involvement of protein kinases (PKA, PKC and PKB) in nitric oxide (NO)-induced apoptosis with sodium nitroprusside plus N-acetyl-L-cysteine in the IPLB-LdFB cell line from the insect Lymantria dispar was investigated. The presence of protein kinase-like molecules was demonstrated by western blot analysis. The role of the kinases in programmed cell death was analysed in cytofluorimetric experiments by incubating the insect cells with H-89 (a specific inhibitor of PKA), calphostin C (an inhibitor of PKC) or wortmannin (an inhibitor of phosphatidylinositol 3-kinase). The results show that PKA is correlated with the induction and PKC and PKB with the prevention of NO-induced insect cell death. Moreover, NO-induced apoptosis involves the release of cytochrome c.
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Received 15 March 2002; accepted 25 March 2002
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Malagoli, D., Conte, A. & Ottaviani, E. Protein kinases mediate nitric oxide-induced apoptosis in the insect cell line IPLB-LdFB. CMLS, Cell. Mol. Life Sci. 59, 894–901 (2002). https://doi.org/10.1007/s00018-002-8476-9
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DOI: https://doi.org/10.1007/s00018-002-8476-9