Abstract:
Objective and design: This study investigated whether prostaglandins (PGs) are involved in 5-hydroxytryptamine (5-HT)-induced synovial plasma extravasation.¶Materials and methods: Male Sprague-Dawley rat knee joints were perfused with 5-HT and synovial capillary Evans Blue dye leakage was measured using spectrophotometry. Cyclooxygenase (COX) inhibitors and PG receptor subtype-selective antagonists were tested for the ability to reduce 5-HT-induced synovial plasma extravasation.¶Results: 5-HT-induced plasma extravasation was inhibited by indomethacin. The COX-1 selective inhibitor SC-560 and the COX-2 selective inhibitor NS-398 were equally effective, indicating that both isoforms are involved. Antagonists selective for EP1, EP2 and DP receptor subtypes significantly attenuated the 5-HT-induced plasma extravasation. However, antagonists selective for FP, IP and TP subtypes failed to reduce 5-HT-induced plasma extravasation.¶Conclusions: These results demonstrate that multiple, but selective, subtypes of PGs mediate synovial plasma extravasation produced by 5-HT, and suggest that PGs act downstream of 5-HT in the inflammatory cascade.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received 10 July 2002; returned for revision 10 September 2002; accepted by G. Letts 27 September 2002
RID="*"
ID="*"These authors contributed equally to this work.
RID="*"
RID="**"
ID="**"Correspondence to: P. Pierce Palmer
Rights and permissions
About this article
Cite this article
Xie, G., Wang, Y., Sharma, M. et al. 5-Hydroxytryptamine-induced plasma extravasation in the rat knee joint is mediated by multiple prostaglandins. Inflamm. res. 52, 032–038 (2003). https://doi.org/10.1007/s000110300011
Issue Date:
DOI: https://doi.org/10.1007/s000110300011