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Inflammation Research

, Volume 50, Issue 2, pp 96–101 | Cite as

Inhibition of 5-lipoxygenase activity by the natural anti-inflammatory compound aethiopinone

  • R. Benrezzouk
  • M.C. Terencio
  • M.L. Ferrandiz
  • M. Hernandez-Perez
  • R. Rabanal
  • M.J. Alcaraz

Abstract:

Objetive and Design: We have investigated the mechanisms of action of aethiopinone, an anti-inflammatory compound from Salvia aethiopis L. roots.¶Material and Subjects: Human neutrophils from healthy volunteers and murine peritoneal macrophages. Swiss mice were randomly divided into groups of six animals.¶Treatment: Test compounds were applied topically in the mouse ear oedema test. In the air pouch, mice received aethiopinone (0.001-0.5 μmol/pouch or 12.5-50 mg/kg p.o.).¶Methods: LTB4 production was assayed in human neutrophils and COX-2 and iNOS activities in murine macrophages. Air pouches were induced subcutaneously in mice and injected with zymosan on the day six. Mouse ear oedema was induced by arachidonic acid. Dunnett's t-test was employed for statistical analysis.¶Results: We have observed potent inhibitory effects on human neutrophil LTB4 production without effects on COX or NOS activities. Aethiopinone is an in vitro inhibitor of 5-LO from human neutrophils (IC50 = 0.11 μM). In addition, aethiopinone reduced leukocyte accumulation and showed in vivo inhibitory activity on this enzyme.¶Conclusions: Our results indicate that inhibition of 5-LO could participate in the anti-inflammatory properties of this natural product.¶

Key words: Aethiopinone; 5-lipoxygenase; Air pouch; Ear oedema. 

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Copyright information

© Birkhäuser Verlag, 2001

Authors and Affiliations

  • R. Benrezzouk
    • 1
  • M.C. Terencio
    • 1
  • M.L. Ferrandiz
    • 1
  • M. Hernandez-Perez
    • 2
  • R. Rabanal
    • 2
  • M.J. Alcaraz
    • 1
  1. 1.Department of Pharmacology, University of Valencia, Faculty of Pharmacy, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain, Fax 34-96-3864292, e-mail: maria.j.alcaraz@uv.es ES
  2. 2.Department of Physical Medicine and Pharmacology, University of La Laguna, 38206 Tenerife, Spain ES

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