Skip to main content

Advertisement

SpringerLink
Log in

NLRP12 negatively modulates inducible nitric oxide synthase (iNOS) expression and tumor necrosis factor-α production in Porphyromonas gingivalis LPS-treated mouse macrophage cell line (RAW264.7)

  • Short Communication
  • Published:
Inflammation Research Aims and scope Submit manuscript

Abstract

Objective

The aim of the present study is to investigate the participation of NLRP12 in Porphyromonas gingivalis LPS-activated mouse macrophages.

Methods

NLRP12-depleted mouse macrophages were stimulated with P. gingivalis LPS (1 μg/ml.). At indicated time points, the treated cells were lysed and the supernatant from treated cells was collected. Gene and protein expression of NLRP12 and iNOS were determined by RT-PCR and immunoblotting, respectively. The level of TNF-α production in the supernatant of the activated cells was determined by ELISA.

Results and conclusion

NLRP12 was upregulated in response to stimulation with P. gingivalis LPS. In addition, when NLRP12 was depleted in P. gingivalis LPS-treated macrophages, an increase in TNF-α production and iNOS expression were observed when compared to those of the control cells, indicating that NLRP12 downregulates the inflammatory cytokine and antimicrobial molecule production in the macrophages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. How KY, Song KP, Chan KG. Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum Line. Front Microbiol. 2016;7:53. https://doi.org/10.3389/fmicb.2016.00053.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Hajishengallis G, Lamont RJ. Breaking bad: manipulation of the host response by Porphyromonas gingivalis. Eur J Immunol. 2014;44(2):328–38. https://doi.org/10.1002/eji.201344202.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Zhang J, Yu C, Zhang X, Chen H, Dong J, Lu W, et al. Porphyromonas gingivalis lipopolysaccharide induces cognitive dysfunction, mediated by neuronal inflammation via activation of the TLR4 signaling pathway in C57BL/6 mice. J Neuroinflammation. 2018;15(1):37. https://doi.org/10.1186/s12974-017-1052-x.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Tuncer S, Fiorillo MT, Sorrentino R. The multifaceted nature of NLRP12. J Leukoc Biol. 2014;96(6):991–1000. https://doi.org/10.1189/jlb.3RU0514-265RR.

    Article  CAS  PubMed  Google Scholar 

  5. Allen IC, McElvania-TeKippe E, Wilson JE, Lich JD, Arthur JC, Sullivan JT, et al. Characterization of NLRP12 during the in vivo host immune response to Klebsiella pneumoniae and Mycobacterium tuberculosis. PLoS One. 2013;8(4):e60842. https://doi.org/10.1371/journal.pone.0060842.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Vladimer GI, Weng D, Paquette SW, Vanaja SK, Rathinam VA, Aune MH, et al. The NLRP12 inflammasome recognizes Yersinia pestis. Immunity. 2012;37(1):96–107. https://doi.org/10.1016/j.immuni.2012.07.006.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Zaki MH, Man SM, Vogel P, Lamkanfi M, Kanneganti TD. Salmonella exploits NLRP12-dependent innate immune signaling to suppress host defenses during infection. Proc Natl Acad Sci USA. 2014;111(1):385–90. https://doi.org/10.1073/pnas.1317643111.

    Article  CAS  PubMed  Google Scholar 

  8. Deng QC, Deng CT, Li WS, Shu SW, Zhou MR, Kuang WB. NLRP12 promotes host resistance against Pseudomonas aeruginosa keratitis inflammatory responses through the negative regulation of NF-kappaB signaling. Eur Rev Med Pharmacol Sci. 2018;22(23):8063–75. https://doi.org/10.26355/eurrev_201812_16496.

    Article  PubMed  Google Scholar 

  9. Pudla M, Kulsantiwong P, Srisaowakarn C, Utaisincharoen P. Regulation of sterile alpha- and armadillo motif (SARM) containing protein expression in Pam2CSK4- and Pam3CSK4-activated mouse macrophage cell line (RAW264.7) requires TLR9. Inflamm Res. 2017;66(12):1099–105. https://doi.org/10.1007/s00011-017-1090-y.

    Article  CAS  PubMed  Google Scholar 

  10. Ullah MO, Sweet MJ, Mansell A, Kellie S, Kobe B. TRIF-dependent TLR signaling, its functions in host defense and inflammation, and its potential as a therapeutic target. J Leukoc Biol. 2016;100(1):27–45. https://doi.org/10.1189/jlb.2RI1115-531R.

    Article  CAS  PubMed  Google Scholar 

  11. Hajishengallis G, Krauss JL, Liang S, McIntosh ML, Lambris JD. Pathogenic microbes and community service through manipulation of innate immunity. Adv Exp Med Biol. 2012;946:69–85. https://doi.org/10.1007/978-1-4614-0106-3_5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Taira TM, Lima V, Prado DS, Silva TA, Issa JPM, da Silva LAB, et al. NLRP12 attenuates inflammatory bone loss in experimental apical periodontitis. J Dent Res. 2019;98(4):476–84. https://doi.org/10.1177/0022034518820289.

    Article  CAS  PubMed  Google Scholar 

  13. Silveira TN, Gomes MT, Oliveira LS, Campos PC, Machado GG, Oliveira SC. NLRP12 negatively regulates proinflammatory cytokine production and host defense against Brucella abortus. Eur J Immunol. 2017;47(1):51–9. https://doi.org/10.1002/eji.201646502.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by Thailand Research Fund (Grant number MRG6180303) for support of MP.

Author information

Authors and Affiliations

  1. Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, 10400, Thailand

    Matsayapan Pudla

  2. Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Chanya Srisaowakarn & Pongsak Utaisincharoen

Authors
  1. Matsayapan Pudla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chanya Srisaowakarn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pongsak Utaisincharoen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Matsayapan Pudla.

Ethics declarations

Conflict of interest

The authors declare no potential conflicts of interest with respect to authorship and/or publication of this article.

Additional information

Responsible Editor: John Di Battista.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pudla, M., Srisaowakarn, C. & Utaisincharoen, P. NLRP12 negatively modulates inducible nitric oxide synthase (iNOS) expression and tumor necrosis factor-α production in Porphyromonas gingivalis LPS-treated mouse macrophage cell line (RAW264.7). Inflamm. Res. 68, 841–844 (2019). https://doi.org/10.1007/s00011-019-01267-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00011-019-01267-3

Keywords

Search

Navigation