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Associations of VCAM-1 gene polymorphisms with obesity and inflammation markers

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Abstract

Objective

Among the inflammatory mediators involved in the pathogenesis of obesity, cell adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) stand out. The aim of this study was to investigate the associations of ICAM-1 and VCAM-1 gene variants with obesity and to investigate the associations between these genetic polymorphisms and CRP, UA, and WBC count.

Method

Four SNPs of the VCAM-1 gene (rs3176860, rs2392221, rs3917010 and rs3176879) and two SNPs of the ICAM-1 gene (rs281432 and rs5498) were analyzed in 181 control (18 < BMI < 23) and 144 obese (BMI ≥ 25) subjects. The SNPs were genotyped by direct sequencing.

Results

In allele frequency analysis, the G allelic frequency of rs3176860 in the VCAM-1 gene was lower in the obese group (30.9%) than in the controls (41.2%) (P = 0.007). The C allelic frequency of rs3917010 was lower in the obese group (18.1%) than in the control (25.1%) (P = 0.03). In the haplotype analysis of VCAM-1 gene, the ht1 (ACA) was higher and ht2 (GCC) was lower in the obese subjects than in the controls (P = 0.0057 and P = 0.037, respectively). In the obese group, participants carrying the G allele of rs3176860 of the VCAM-1 gene showed a higher percentage of segmented neutrophils and CRP levels than those carrying only the A allele (P = 0.028 and P = 0.042, respectively).

Conclusions

The results of this study suggest that VCAM-1 gene variants may be related to obesity and inflammatory markers in the Korean population.

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Abbreviations

BMI:

Body Mass Index

CRP:

C-reactive protein

SBP:

Systolic blood pressures

DBP:

Diastolic blood pressures

FBS:

Fasting plasma glucose

HBA1C:

Hemoglobin A1C

TG:

Triglycerides

TC:

Total cholesterol

LDL:

Low-density lipoprotein

HDL:

High-density lipoprotein

WBC:

White blood cells

NEUT:

Neutrophil percentage

LYMPH:

Lymphatic cell percentage

MONO:

Mononuclear cell percentage

SNP:

Single nucleotide polymorphism

MAF:

Minor allele frequency

SD:

Standard deviation

OR:

Odds ratios

95% CI:

95% confidence intervals

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Govrnment (MSIP) (No. 2014R1A5A2010008).

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Authors and Affiliations

  1. Department of Preventive Medicine, School of Medicine, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Korea

    Gyeong Im Yu & Dong Hoon Shin

  2. College of Nursing, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Korea

    Sang Eun Jun

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  1. Gyeong Im Yu
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Correspondence to Dong Hoon Shin.

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Responsible Editor: John Di Battista.

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Yu, G.I., Jun, S.E. & Shin, D.H. Associations of VCAM-1 gene polymorphisms with obesity and inflammation markers. Inflamm. Res. 66, 217–225 (2017). https://doi.org/10.1007/s00011-016-1006-2

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  • DOI: https://doi.org/10.1007/s00011-016-1006-2

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