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HDAC9, TWIST1 and FERD3L gene expression in asymptomatic stable and unstable carotid plaques

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Abstract

Background and objectives

A variant located at the end of HDAC9 gene within clusters of DNAse I sensitivity zones and histone modification hotspots has been associated with large vessel stroke and could be linked to plaque instability. The aim of the study is to define if an altered expression of HDAC9, TWIST1 and FERD3L genes could be involved in plaque vulnerability.

Methods

Histological classification and gene expression analysis were performed in 6 stable and 16 unstable plaques obtained from asymptomatic patients undergoing endarterectomy. Gene expression was analysed by real-time PCR.

Results and conclusions

TWIST1 gene expression resulted higher in stable plaques (P < 0.02). HDAC9 gene expression followed a similar trend (P = 0.11). These results highlighting the significant correlation between TWIST and HDAC9 gene expression suggest that both genes may contribute to plaque stability in a coordinated way.

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Fig. 1

Abbreviations

ASP:

Asymptomatic stable plaques

AUP:

Asymptomatic unstable plaque

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Acknowledgments

This work was supported by AIRC-Cariverona Romanelli and F.U.R. 2013–2014 Gomez Lira.

Author contribution

SF and MG: performed the experiments. SF and MGL: manuscript preparation. AS: patients recruitment, surgical excision of plaques and blood samples collection; SO: clinical data collection. MGL: research study design. MGL, MGR and AS: contributed with essential reagents and tools. SF and GM performed data analysis. CS: study supervisor. MGR: manuscript revision. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Department of Neurological, Biomedical and Movement Sciences, Section of Biology and Genetics, University of Verona, Strada Le Grazie, 8, 37134, Verona, Italy

    Silvia Ferronato, Matteo Gelati, Silvia Olivato, Giovanni Malerba, Maria Grazia Romanelli & Macarena Gomez-Lira

  2. Department of Surgery, University of Verona, Verona, Italy

    Alberto Scuro

  3. Division of Vascular Surgery, University of Siena, Siena, Italy

    Carlo Setacci

Authors
  1. Silvia Ferronato
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  2. Matteo Gelati
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  3. Alberto Scuro
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  4. Silvia Olivato
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  5. Giovanni Malerba
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  6. Maria Grazia Romanelli
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  7. Macarena Gomez-Lira
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  8. Carlo Setacci
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Corresponding author

Correspondence to Maria Grazia Romanelli.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The study was approved by the Ethical Committee of the University Hospital.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Responsible Editor: John Di Battista.

Silvia Ferronato and Matteo Gelati are both first authors.

Macarena Gomez Lira and Carlo Setacci are both co-senior authors.

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Supplementary material 1 (DOCX 20 KB)

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Ferronato, S., Gelati, M., Scuro, A. et al. HDAC9, TWIST1 and FERD3L gene expression in asymptomatic stable and unstable carotid plaques. Inflamm. Res. 65, 261–263 (2016). https://doi.org/10.1007/s00011-015-0904-z

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  • DOI: https://doi.org/10.1007/s00011-015-0904-z

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