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Anti-angiogenic and anti-inflammatory effect of Magnolol in the oxygen-induced retinopathy model

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Abstract

Objective

In the present study, we investigated the effects of Magnolol on the retinal neovascularization (RNV) and local glial cells in an oxygen-induced retinopathy (OIR) model and explored their molecular mechanisms.

Materials and methods

Neonatal C57BL/6J mice were subjected to 75 % O2 ± 5 % from postnatal day (P) 7 to P12 and subsequently returned to room air. Mice were injected with 25 mg/kg Magnolol intraperitoneally once a day from P12 to P17, then retinas were harvested and flat-mounted to assess the retinal vessels, astrocytes and microglia. To clarify the molecular mechanisms of Magnolol, we observed the level of inflammatory cytokines such as interleukin (IL)-1β, IL-6, monocyte chemoattractant protein-1, tumor necrosis factor-α, and analyzed the hypoxia-inducible factor (HIF)-1α/vascular endothelial growth factor (VEGF) pathway in OIR mice.

Results

Intraperitoneal administration of Magnolol resulted in significant reduction of RNV without retinal toxicity or perturbation of developmental retinal angiogenesis. In addition, Magnolol preserved the astrocyte morphology and diminished the activation of microglia. Moreover, Magnolol down regulated the expression of inflammatory cytokines and inactivated the HIF-1α/VEGF pathway.

Conclusions

These results indicated that Magnolol might have potential for the treatment of pathological retinal angiogenesis and glial dysfunctions via anti-inflammation and inhibition of HIF-1α/VEGF pathway.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81470030).

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    Authors and Affiliations

    1. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510000, Guangdong Province, People’s Republic of China

      Boyu Yang, Yue Xu, Shanshan Yu, Yongsheng Huang, Lin Lu & Xiaoling Liang

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    1. Boyu Yang
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    2. Yue Xu
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    3. Shanshan Yu
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    Correspondence to Xiaoling Liang.

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    Responsible Editor: John Di Battista.

    Boyu Yang and Yue Xu have contributed equally to this work.

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    Yang, B., Xu, Y., Yu, S. et al. Anti-angiogenic and anti-inflammatory effect of Magnolol in the oxygen-induced retinopathy model. Inflamm. Res. 65, 81–93 (2016). https://doi.org/10.1007/s00011-015-0894-x

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    • DOI: https://doi.org/10.1007/s00011-015-0894-x

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