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Effects of pre- and post-treatment with plant polyphenols on human keratinocyte responses to solar UV

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Abstracts

Background

The understanding of the anti-inflammatory mechanisms of action of plant polyphenols (PPs) and clarification of the relationship between their anti-inflammatory and antioxidant properties may result in a new therapeutic approach to skin cancers.

Objective

To elucidate the underlying mechanism, we analyzed the ability of PPs to attenuate inflammatory, metabolic and oxidative cellular responses to UV irradiation.

Methods

Normal human epidermal keratinocytes (NHEK) were exposed to physiologically relevant dose of solar-simulated UV irradiation. Effects of pre- and post-treatment with PPs on the overproduction of peroxides and inflammatory mediators (mRNA and protein) were analyzed using real-time RT-PCR, enzyme-linked immunosorbent and fluorometric techniques.

Results

Differences between the effectiveness of pre- and post-treatment with polyphenols was found. In particular, PPs post-treatment, but not pretreatment, completely abolished overexpression of Cyp1a1 and Cyp1b1 genes and elevation of intracellular peroxides in NHEK irradiated by UV. Post-treatment with PPs also more efficiently than pretreatment prevented UV-induced overexpression of IL-1 beta, IL-6 and COX2 mRNAs.

Conclusion

Our data strongly suggest that PPs predominantly affect delayed molecular and cellular events initiated in NHEK by solar UV rather than primary photochemical reactions. PPs may be important component in cosmetic formulations for post-sun skin care.

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References

  1. De Gruijl FR. Skin cancer and solar UV radiation. Eur J Cancer. 1999;35:2003–9.

    Article  PubMed  Google Scholar 

  2. Aitken GR, Henderson JR, Chang SC, McNeil CJ, Birch-Machin MA. Direct monitoring of UV-induced free radical generation in HaCaT keratinocytes. Clin Exp Dermatol. 2007;32:722–7.

    Article  PubMed  CAS  Google Scholar 

  3. Valencia A, Kochevar IE. Nox1-based NADPH oxidase is the major source of UVA-induced reactive oxygen species in human keratinocytes. J Invest Dermatol. 2008;128:214–22.

    Article  PubMed  CAS  Google Scholar 

  4. Sachsenmaier C, Radler-Pohl A, Muller A, Herrlich P, Rahmsdorf HJ. Damage to DNA by UV light and activation of transcription factors. Biochem Pharmacol. 1994;47:129–36.

    Article  PubMed  CAS  Google Scholar 

  5. Grether-Beck S, Olaizola-Horn S, Schmitt H, Grewe M, Jahnke A, Johnson JP, Briviba K, Sies H, Krutmann J. Activation of transcription factor AP-2 mediates UVA radiation and singlet oxygen-induced expression of the human intercellular adhesion molecule 1 gene. Proc Natl Acad Sci USA. 1996;93:14586–91.

    Article  PubMed  CAS  Google Scholar 

  6. Mahns A, Wolber R, Stab F, Klotz LO, Sies H. Contribution of UVB and UVA to UV-dependent stimulation of cyclooxygenase-2 expression in artificial epidermis. Photochem Photobiol Sci. 2004;3:257–62.

    Article  PubMed  CAS  Google Scholar 

  7. Belli R, Amerio P, Brunetti L, Orlando G, Toto P, Proietto G, Vacca M, Tulli A. Elevated 8-isoprostane levels in basal cell carcinoma and in UVA irradiated skin. Int J Immunopathol Pharmacol. 2005;18:497–502.

    PubMed  CAS  Google Scholar 

  8. Pastore S, Mascia F, Mariani V, Girolomoni G. The epidermal growth factor receptor system in skin repair and inflammation. J Invest Dermatol. 2008;128:1365–74.

    Article  PubMed  CAS  Google Scholar 

  9. Korkina LG, Pastore S, De Luca C, Kostyuk VA. Metabolism of plant polyphenols in the skin: beneficial versus deleterious effects. Curr Drug Metab. 2008;9:10–29.

    Article  Google Scholar 

  10. Klebanoff SJ. Myeloperoxidase-halide-hydrogen peroxide antibacterial system. J Bacteriol. 1968;95:2131–8.

    PubMed  CAS  Google Scholar 

  11. Adams DO, Hamilton TA. Macrophages as destructive cells in host defense. In: Gallin JI, Goldstein IM, Synderman R, editors. The Inflammation: basic principles and clinical correlates. New York: Raven Press; 1992. pp. 637–662.

  12. Baumann H, Gauldie J. The acute phase response. Immunol Today. 1994;15:74–80.

    Article  PubMed  CAS  Google Scholar 

  13. Shacter E, Beecham EJ, Covey JM, Kohn KW, Potter M. Activated neutrophils induce prolonged DNA damage in neighboring cells. Carcinogenesis. 1988;9:2297–304.

    Article  PubMed  CAS  Google Scholar 

  14. Brigelius-Flohé R, Flohé L. Basic principles and emerging concepts in the redox control of transcription factors. Antioxid Redox Signal. 2011;15:2335–81.

    Article  PubMed  Google Scholar 

  15. Tunon MJ, Garcia-Mediavilla MV, Sanchez-Campos S, Gonzalez-Gallego J. Potential of flavonoids as anti-inflammatory agents: modulation of pro-inflammatory gene expression and signal transduction pathways. Curr Drug Metab. 2009;10:256–71.

    Article  PubMed  CAS  Google Scholar 

  16. Vanden Berghe W, Haegeman G. Epigenetic remedies by dietary phytochemicals against inflammatory skin disorders: myth or reality? Curr Drug Metab. 2010;11:436–50.

    Article  PubMed  CAS  Google Scholar 

  17. Nichols JA, Katiyar SK. Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res. 2010;302:71–83.

    Article  PubMed  CAS  Google Scholar 

  18. Liu ML, Yu LC. Potential protection of green tea polyphenols against ultraviolet irradiation-induced injury on rat cortical neurons. Neurosci Lett. 2008;444:236–9.

    Article  PubMed  CAS  Google Scholar 

  19. Wu LY, Zheng XQ, Lu JL, Liang YR. Protective effect of green tea polyphenols against ultraviolet B-induced damage to HaCaT cells. Hum Cell. 2009;22:18–24.

    Article  PubMed  Google Scholar 

  20. Vicentini FTMC, He T, Shao Y, Fonseca MJV, Verri WA, Fisher GJ, Xu Y. Quercetin inhibits UV irradiation-induced inflammatory cytokine production in primary human keratinocytes by suppressing NF-kB pathway. J Dermatol Sci. 2011;61:162–8.

    Article  PubMed  CAS  Google Scholar 

  21. Pastore S, Potapovich A, Lulli D, Fidanza P, Kostyuk V, De Luca C, Mikhal’chik E, Korkina L. Plant polyphenols regulate chemokine expression and tissue repair in human keratinocytes through interaction with cytoplasmic and nuclear components of epidermal growth factor receptor (EGFR) system. Antioxid Redox Signal. 2012;16:314–28.

    Article  PubMed  CAS  Google Scholar 

  22. Pastore S, Lulli D, Potapovich AI, Fidanza P, Kostyuk VA, Dellambra E, de Luca C, Maurelli R, Korkina LG. Differential modulation of stress-inflammation responses by plant polyphenols in cultured normal human keratinocytes and immortalized HaCaT cells. J Dermatol Sci. 2011;63:104–14.

    PubMed  CAS  Google Scholar 

  23. Adhami VM, Afaq F, Ahmad N. Suppression of ultraviolet B exposure-mediated activation of NFkappaB in normal human keratinocytes by resveratrol. Neoplasia. 2003;5:74–82.

    PubMed  CAS  Google Scholar 

  24. Kojima H, Nakatsubo N, Kikuchi K, Kawahara S, Kirino Y, Nagoshi H, Hirata Y, Nagano T. Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins. Anal Chem. 1998;70:2446–53.

    Article  PubMed  CAS  Google Scholar 

  25. Kooy NW, Royall JA, Ischiropoulos H, Beckman JS. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med. 1994;16:149–56.

    Article  PubMed  CAS  Google Scholar 

  26. Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci USA. 1990;87:1620–4.

    Article  PubMed  CAS  Google Scholar 

  27. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25:402–8.

    Article  PubMed  CAS  Google Scholar 

  28. Kostyuk VA, Potapovich AI, Lulli D, Stancato A, De Luca C, Pastore S, Korkina L. Modulation of human keratinocyte responses to solar UV by plant polyphenols as a basis for chemoprevention of non-melanoma skin cancers. Curr Med Chem. 2013;20:869–79.

    Google Scholar 

  29. Afaq F, Katiyar SK. Polyphenols: skin photoprotection and inhibition of photocarcinogenesis. Mini Rev Med Chem. 2011;11:1200–15.

    PubMed  CAS  Google Scholar 

  30. Wu S, Wang L, Jacoby AM, Jasinski K, Kubant R, Malinski T. Ultraviolet B light-induced nitric oxide/peroxynitrite imbalance in keratinocytes-implications for apoptosis and necrosis. Photochem Photobiol. 2010;86:389–96.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The work was partly financed by the grant from Italian Ministry for Health (RC. IDI IRCCS-2012).

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Correspondence to Vladimir A. Kostyuk.

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Responsible Editor: Helen Griffiths.

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Potapovich, A.I., Kostyuk, V.A., Kostyuk, T.V. et al. Effects of pre- and post-treatment with plant polyphenols on human keratinocyte responses to solar UV. Inflamm. Res. 62, 773–780 (2013). https://doi.org/10.1007/s00011-013-0634-z

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  • DOI: https://doi.org/10.1007/s00011-013-0634-z

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