Inflammation Research

, Volume 62, Issue 5, pp 499–506 | Cite as

Protective effect of gossypol on lipopolysaccharide-induced acute lung injury in mice

  • Zhicheng Liu
  • Zhengtao Yang
  • Yunhe Fu
  • Fenyang Li
  • Dejie Liang
  • Ershun Zhou
  • Xiaojing Song
  • Wen Zhang
  • Xichen Zhang
  • Yongguo Cao
  • Naisheng Zhang
Original Research Paper



Gossypol has been reported to have anti-inflammatory properties. The purpose of this study was to evaluate the effect of gossypol on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.


Male BALB/c mice were pretreated with gossypol 1 h before intranasal instillation of LPS. Then, 7 h after LPS administration, the myeloperoxidase in histology of lungs, lung wet/dry ratio and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in the BALF were measured by ELISA. The extent of phosphorylation of IκB-α, p65 NF-κB, p46–p54 JNK, p42–p44 ERK, and p38 were detected by western blot.


Gossypol markedly attenuated the LPS-induced histological alterations in the lung and inhibited the production of TNF-α, IL-1β and IL-6. Additionally, gossypol reduced the inflammatory cells in BALF, decreased the wet/dry ratio of lungs and inhibited the phosphorylation of IκB-α, p65 NF-κB, p46–p54 JNK, p42–p44 ERK, and p38 caused by LPS.


The data suggest that anti-inflammatory effects of gossypol against the LPS-induced ALI may be due to its ability of inhibition of the NF-κB and MAPKs signaling pathways. Gossypol may be a promising potential therapeutic reagent for ALI treatment.


Gossypol Lipopolysaccharide (LPS) Acute lung injury (ALI) Nuclear factor-kappa B (NF-κB) Mitogen-activated protein kinases (MAPKs) 



This work was supported by a grant from the National Natural Science Foundation of China (No. 30972225, 30771596) and the Research Fund for the Doctoral Program of Higher Education of China (No. 20110061130010).


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Copyright information

© Springer Basel 2013

Authors and Affiliations

  • Zhicheng Liu
    • 1
  • Zhengtao Yang
    • 1
  • Yunhe Fu
    • 1
  • Fenyang Li
    • 1
  • Dejie Liang
    • 1
  • Ershun Zhou
    • 1
  • Xiaojing Song
    • 1
  • Wen Zhang
    • 1
  • Xichen Zhang
    • 1
  • Yongguo Cao
    • 1
  • Naisheng Zhang
    • 1
  1. 1.Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary MedicineJilin UniversityChangchunPeople’s Republic of China

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