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Increased synovial expression of IL-27 by IL-17 in rheumatoid arthritis

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Abstract

Objectives and design

Interleukin (IL) 17 plays an important role in synovial inflammation and bone destruction in rheumatoid arthritis (RA), while IL-27 exerts a regulatory role in T cell-mediated immune responses. Our aim was to study the influence of IL-17 on IL-27 production in RA.

Materials and methods

Following injection of IL-17 in the knee joint of CIA mice, synovium was examined for IL-17 and IL-27 expression by western blot, real-time PCR, and immunohistochemistry. IL-17 and IL-27 levels were measured by ELISA in mouse bone marrow-derived dendritic cells (BM-DCs) and in synovial fluid (SF) macrophages from RA patients.

Results

IL-17 exacerbated disease progression in CIA mice. Histological analysis showed increased pannus formation associated with cartilage and bone erosion following injection with IL-17. The expression of IL-27 was increased in CIA mice. The expression of IL-17 and IL-27 was increased more in IL-17-injected CIA mice than in control mice. The majority of cells expressing IL-27 were co-localized with synovial macrophages. Increased expression of IL-27 by application of recombinant IL-17 was confirmed in CIA BM-DCs and in SF macrophages from RA patients.

Conclusion

IL-17 enhanced expression of IL-27 in synovial macrophages from RA patients and CIA mice, indicating an interaction between IL-17 and IL-27 as an autoregulatory mechanism.

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Abbreviations

RA:

Rheumatoid arthritis

CIA:

Collagen-induced arthritis

IL-17:

Interleukin 17

IL-27:

Interleukin 27

DCs:

Dendritic cells

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Acknowledgments

This study was supported by a 2011 research grant from Pusan National University Yangsan Hospital, a National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (2011-0009020).

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Authors and Affiliations

  1. Medical Research Center for Ischemic Tissue Regeneration, School of Medicine, Pusan National University, Yangsan-si, Gyeongsangnam-do, 626-770, Republic of Korea

    Chi Dae Kim & So Youn Park

  2. Department of Internal Medicine, School of Medicine, Pusan National University, Yangsan-si, Gyeongsangnam-do, Korea

    Seung Hoon Baek, Seung Geun Lee & Young Eun Park

  3. Department of Internal Medicine, College of Medicine, Kosin University, Busan, Korea

    Geun Tae Kim

Authors
  1. Seung Hoon Baek
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  2. Seung Geun Lee
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  3. Young Eun Park
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  4. Geun Tae Kim
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  5. Chi Dae Kim
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  6. So Youn Park
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Correspondence to So Youn Park.

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Responsible Editor: John Di Battista.

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Baek, S.H., Lee, S.G., Park, Y.E. et al. Increased synovial expression of IL-27 by IL-17 in rheumatoid arthritis. Inflamm. Res. 61, 1339–1345 (2012). https://doi.org/10.1007/s00011-012-0534-7

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  • DOI: https://doi.org/10.1007/s00011-012-0534-7

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