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The effect of sRAGE-Fc fusion protein attenuates inflammation and decreases mortality in a murine cecal ligation and puncture model

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Abstract

Objective

Inhibition of the receptor for advanced glycation end products (RAGE) may attenuate the systemic inflammatory response and ensuing severe sepsis. We report an investigation into the effect of soluble RAGE (sRAGE)-Fc fusion protein in severe sepsis induced by a cecal ligation and puncture (CLP) procedure.

Materials and methods

The experiment was performed using CLP control mice, mice treated with 0.5 or 1.0 μg sRAGE-Fc fusion protein, and sham surgery mice.

Results

Survival benefits over the CLP control group were evident (P = 0.036) in mice given 1.0 μg sRAGE-Fc fusion protein. In addition, the pulmonary inflammation score in the sRAGE-Fc fusion protein-treated group was significantly lower than that in the CLP control group (P < 0.05). Lung tissue in the sRAGE-Fc fusion protein-treated group revealed a significant decrease in the expression of inflammatory cytokines. Furthermore, levels of interleukin (IL)-1β and tumor necrosis factor-α were significantly lower in sRAGE-Fc fusion protein treated groups (P < 0.001). Moreover, IL-6 levels showed a significant difference between CLP control and sRAGE-Fc fusion protein treated groups (P < 0.01).

Conclusions

sRAGE-Fc fusion protein has beneficial effects in a standard murine model of polymicrobial, intra-abdominal severe sepsis.

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Acknowledgments

This work was supported by Yonsei University Research Fund of 2011.

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Authors and Affiliations

  1. Department of Internal Medicine and AIDS Research Institute, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea

    Su Jin Jeong, Hye Won Kim, Nam Su Ku, Sang Hoon Han, Chang Oh Kim, Jun Yong Choi, Young Goo Song & June Myung Kim

  2. Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea

    Beom Jin Lim

  3. Division of Cardiology, Cardiovascular Center, Yonsei University College of Medicine, Seoul, Republic of Korea

    Sungha Park & Donghoon Choi

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  1. Su Jin Jeong
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Correspondence to Sang Hoon Han.

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Responsible Editor: Artur Bauhofer.

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Jeong, S.J., Lim, B.J., Park, S. et al. The effect of sRAGE-Fc fusion protein attenuates inflammation and decreases mortality in a murine cecal ligation and puncture model. Inflamm. Res. 61, 1211–1218 (2012). https://doi.org/10.1007/s00011-012-0518-7

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  • DOI: https://doi.org/10.1007/s00011-012-0518-7

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