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Pro-inflammatory cytokine interleukin-1β promotes the development of intestinal stem cells

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Abstract

Objective

We investigated the effect of IL-1β on the development of intestinal epithelial stem cells.

Materials and methods

Normal intestinal epithelial cell line IEC-18 cells were cultured in the presence or absence of 200 pM of IL-1β in serum-free medium (SFM) for various time periods. The effects of IL-1β on intestinal stem cell self-renewal and IEC-18 cell proliferation were evaluated by a colony formation assay, MTT assay, and a focus formation assay. The expression of stemness genes including Bmi-1, Lgr-5, c-myc, Nanog, and β-catenin in IEC-18 cells were measured by quantitative PCR and western blot analysis.

Results

IEC-18 cells grew as a monolayer in SFM in the absence of IL-1β. Cellular spheres were formed when IEC-18 cells were grown in SFM in the presence of IL-1β. IL-1β induced the development of large colonies in the soft-agar as well as the formation of foci when IEC-18 cells were cultured in type-I collagen-coated plates. The expression of Bmi-1, Lgr-5, c-myc, Nanog, and β-catenin were significantly increased in IEC-18 cells treated with IL-1β.

Conclusion

Our studies provide direct evidence the IL-1β may play an important role in the self-renewal of intestinal epithelial stem cells and the development of cancer stem cells.

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Acknowledgments

We thank Ms. Xiaoxia Wang for her technical help. This research was supported in part by Innovative Research Award (L. Wang) from Johnson Center for Basic Cancer Research at Kansas State University, NIH R21 AI085416 (J. Shi), KBA-CBRI 611310 (J. Shi), NIH NCRR P20-RR017686 (PI: Daniel Marcus; J. Shi).

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Authors and Affiliations

  1. Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA

    Lei Wang, Ziyan Liu, Yijing Li, Loretta Pappan, Amy Galliher-Beckley & Jishu Shi

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  1. Lei Wang
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  2. Ziyan Liu
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  3. Yijing Li
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Correspondence to Jishu Shi.

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Responsible Editor: Liwu Li.

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Wang, L., Liu, Z., Li, Y. et al. Pro-inflammatory cytokine interleukin-1β promotes the development of intestinal stem cells. Inflamm. Res. 61, 1085–1092 (2012). https://doi.org/10.1007/s00011-012-0501-3

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  • DOI: https://doi.org/10.1007/s00011-012-0501-3

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