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Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products

Abstract

Objective and method

Trichomonas vaginalis is a flagellated protozoan parasite that causes human trichomoniasis. Although T. vaginalis itself can secrete lipid mediator leukotriene (LT) B4 leading to neutrophil activation, information regarding the signaling mechanism involved in neutrophil activation induced by T. vaginalis-secreted LTB4 is limited. We investigated whether LTB4 contained in the T. vaginalis-derived secretory products (TvSP) is closely involved in interleukin (IL)-8 production in human neutrophils via LTB4 receptors BLT1 or BLT2.

Results

T. vaginalis produced more than 714 pg/ml of LTB4 per 1 × 107 trichomonads. The ability of trichomonads to secrete LTB4 was inhibited by treatment of trichomonads with the 5-lipo-oxygenease inhibitor AA861, but not the cyclo-oxygenease I inhibitor FR122047. When neutrophils were incubated with TvSP obtained from 1 × 107 trichomonads, IL-8 protein secretion was significantly increased compared to results for cells incubated with medium alone. The stimulatory effect of TvSP on IL-8 production was strongly inhibited by pretreatment of TvSP with lipase, although pretreatment with heat or proteinase K showed little inhibitory effect. Moreover, TvSP-induced IL-8 production was efficiently inhibited when trichomonads were pretreated with AA861 or when neutrophils were pretreated with antagonists for BLT1 or BLT2.

Conclusion

Our results suggest that LTB4 receptors BLT1 and BLT2 are involved in IL-8 production in neutrophils induced by T. vaginalis.

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Acknowledgments

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0021634).

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Correspondence to Myeong Heon Shin.

Additional information

Responsible Editor: Kumar Visvanathan.

Y. H. Nam and A. Min contributed equally to this work.

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Nam, Y.H., Min, A., Kim, S.H. et al. Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products. Inflamm. Res. 61, 97–102 (2012). https://doi.org/10.1007/s00011-011-0425-3

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  • DOI: https://doi.org/10.1007/s00011-011-0425-3

Keywords

  • Trichomonas vaginalis
  • Human neutrophils
  • IL-8
  • Leukotriene B4 receptors BLT1
  • BLT2