Skip to main content
Log in

Increased plasma levels of heparin-binding protein in patients with shock: a prospective, cohort study

  • Original Research Paper
  • Published:
Inflammation Research Aims and scope Submit manuscript

Abstract

Objective

Heparin-binding protein (HBP) is a potent inducer of increased vascular permeability. The purpose of this study was to examine plasma levels of HBP in patients with shock.

Design

Fifty-three consecutive patients with septic and non-septic shock at a mixed-bed intensive care unit were included, as well as 20 age-matched controls. Patients with local infections but without signs of shock served as infectious controls. Enzyme-linked immunosorbent assay was used to determine plasma levels of HBP.

Results

There were no differences in serum HBP levels between healthy controls and those with local infections, including urinary tract infections, pneumonia and gastroenteritis, without shock. Levels of HBP were higher in patients with non-septic shock and septic shock than healthy controls. However, there was no difference in serum HBP levels between patients with septic shock and those with non-septic shock. Moreover, HBP levels were not different between patients with low and high APACHE II scores. Plasma levels of HBP were similar in surviving and non-surviving patients with shock.

Conclusions

HBP is elevated in patients with shock from septic and non-septic etiologies. Future investigations are required to define the functional role of HBP in patients with shock.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Cohen J. The immunopathogenesis of sepsis. Nature. 2002;420:885–91.

    Article  PubMed  CAS  Google Scholar 

  2. Asaduzzaman M, Zhang S, Lavasani S, Wang Y, Thorlacius H. LFA-1 and Mac-1 mediate pulmonary recruitment of neutrophils and tissue damage in abdominal sepsis. Shock. 2008;30:254–9.

    PubMed  Google Scholar 

  3. Rahman M, Zhang S, Chew M, Ersson A, Jeppsson B, Thorlacius H. Platelet-derived CD40L (CD154) mediates neutrophil upregulation of Mac-1 and recruitment in septic lung injury. Ann Surg. 2009;250:783–90.

    Article  PubMed  Google Scholar 

  4. Tapper H, Karlsson A, Mörgelin M, Flodgaard H, Herwald H. Secretion of heparin-binding protein from human neutrophils is determined by its localization in azurophilic granules and secretory vesicles. Blood. 2002;99:1785–93.

    Article  PubMed  CAS  Google Scholar 

  5. Gautam N, Olofsson AM, Herwald H, Iversen LF, Lundgren-Akerlund E, Hedqvist P, Arfors KE, Flodgaard H, Lindbom L. Heparin-binding protein (HBP/CAP37): a missing link in neutrophil-evoked alteration of vascular permeability. Nat Med. 2001;7:1123–7.

    Article  PubMed  CAS  Google Scholar 

  6. Soehnlein O, Zernecke A, Eriksson EE, Rothfuchs AG, Pham CT, Herwald H, Bidzhekov K, Rottenberg ME, Weber C, Lindbom L. Neutrophil secretion products pave the way for inflammatory monocytes. Blood. 2008;112:1461–71.

    Article  PubMed  CAS  Google Scholar 

  7. Chertov O, Michiel DF, Xu L, Wang JM, Tani K, Murphy WJ, Longo DL, Taub DD, Oppenheim JJ. Identification of defensin-1, defensin-2, and CAP37/azurocidin as T-cell chemoattractant proteins released from interleukin-8-stimulated neutrophils. J Biol Chem. 1996;271:2935–40.

    Article  PubMed  CAS  Google Scholar 

  8. Linder A, Christensson B, Herwald H, Björck L, Akesson P. Heparin-binding protein: an early marker of circulatory failure in sepsis. Clin Infect Dis. 2009;49:1044–50.

    Article  PubMed  CAS  Google Scholar 

  9. Bone RC, Balk RA, Cerra FB, Dellinger RP, Fein AM, Knaus WA, Schein RM, Sibbald WJ. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM consensus conference committee. Chest. 1992;101:1644–55.

    Article  PubMed  CAS  Google Scholar 

  10. Dellinger RP, Carlet JM, Masur H, Gerlach H, Calandra T, Cohen J, Gea-Banacloche J, Keh D, Marshall JC, Parker MM, Ramsay G, Zimmerman JL, Vincent JL, Levy MM. Surviving sepsis campaign guidelines for management of severe sepsis and septic shock. Crit Care Med. 2004;32:858–73.

    Article  PubMed  Google Scholar 

  11. Bernard GR, Vincent JL, Laterre PF, LaRosa SP, Dhainaut JF, Lopez-Rodriguez A, Steingrub JS, Garber GE, Helterbrand JD, Ely EW, Fisher CJ Jr. Efficacy and safety of recombinant human activated protein C for severe sepsis. N Engl J Med. 2001;344:699–709.

    Article  PubMed  CAS  Google Scholar 

  12. Knaus WA, Draper EA, Wagner DP, Zimmerman JE. Prognosis in acute organ system failure. Ann Surg. 1985;202:685–93.

    Article  PubMed  CAS  Google Scholar 

  13. Påhlman LI, Mörgelin M, Eckert J, Johansson L, Russell W, Riesbeck K, Soehnlein O, Lindbom L, Norrby-Teglund A, Schumann RR, Björck L, Herwald H. Streptococcal M protein: a multipotent and powerful inducer of inflammation. J Immunol. 2006;177:1221–8.

    PubMed  Google Scholar 

  14. Nakae H, Endo S, Inada K, Takakuwa T, Kasai T. Changes in adhesion molecule levels in sepsis. Res Commun Mol Pathol Pharmacol. 1996;91:329–38.

    PubMed  CAS  Google Scholar 

  15. Silvestre J, Povoa P, Coelho L, Almeida E, Moreira P, Fernandes A, Mealha R, Sabino H. Is CRP a good prognostic marker in septic patients? Int Care Med. 2009;35:909–13.

    Article  CAS  Google Scholar 

  16. Johansson J, Lindbom L, Herwald H, Sjöberg F. Neutrophil-derived heparin binding protein––a mediator of increased vascular permeability after burns? Burns. 2009;35:1185–7.

    Article  PubMed  Google Scholar 

  17. Berkestedt I, Herwald H, Ljunggren L, Nelson A, Bodelsson M. Elevated plasma levels of antimicrobial polypeptides in patients with severe sepsis. J Innate Immun. 2010;2:478–82.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the Swedish Medical Research Council (2007-7480 and 2009-4872), Crafoord foundation, Einar and Inga Nilsson foundation, Harald and Greta Jaensson foundation, Greta and Johan Kock foundation, Fröken Agnes Nilsson foundation, Franke and Margareta Bergqvist foundation, Magnus Bergvall foundation, Mossfelt foundation, Nanna Svartz foundation, Ruth and Richard Julin foundation, Svenska Läkaresällskapet, MAS foundations, Scandinavian Society for Anesthesiology and Intensive Care Acta Foundation, Malmö University Hospital and Lund University. None of these funding bodies were involved in the study design, data collection, analysis, manuscript preparation or submission. We thank Monica Heidenholm for excellent technical assistance.

Conflict of interest

The authors declare that they have no competing interests.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Henrik Thorlacius.

Additional information

Responsible Editor: Artur Bauhofer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chew, M.S., Linder, A., Santen, S. et al. Increased plasma levels of heparin-binding protein in patients with shock: a prospective, cohort study. Inflamm. Res. 61, 375–379 (2012). https://doi.org/10.1007/s00011-011-0422-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00011-011-0422-6

Keywords

Navigation