Inflammation Research

, Volume 60, Issue 9, pp 861–867 | Cite as

Role of heme oxygenase-1 in inflammatory response induced by mechanical stretch in synovial cells

  • Masanobu Takao
  • Toshinori Okinaga
  • Wataru Ariyoshi
  • Kenjiro Iwanaga
  • Ikuo Nakamichi
  • Izumi Yoshioka
  • Kazuhiro Tominaga
  • Tatsuji NishiharaEmail author
Original Research Paper



The purpose of this study was to investigate the mechanism by which heme oxygenase-1 (HO-1) regulates inflammatory responses induced by mechanical stretch in human fibroblast-like synoviocyte (HFLS) cells.

Materials and methods

HFLS cells were cultured in the presence of hemin and seeded into fibronectin-coated silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The genetic expressions of cyclooxygenase-2 (COX-2), interleukin- (IL-) and HO-1 were analyzed using real-time RT-PCR. The expression and localization of HO-1 protein were detected by immunofluorescence staining. The amounts of prostaglandin E2 (PGE2) released into the culture medium were determined using ELISA.


Mechanical stretch enhanced the expressions of COX-2 and IL-1β, and the amount of PGE2 synthesis in HFLS cells, whereas that of HO-1 was slightly increased. In contrast, treatment with hemin enhanced HO-1 gene expression and mechanical stretch enhanced this expression in hemin-pretreated cells. In addition, hemin pretreatment suppressed PGE2 synthesis induced by mechanical stretch.


We found that constitutive HO-1 expression in hemin-pretreated HFLS cells suppressed mechanical stretch-induced inflammatory responses, suggesting that HO-1 may play a role as a regulation factor in synovial tissue inflammation.


Mechanical stretch COX-2 IL-1β HO-1 PGE2 Synovial cell 


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Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  • Masanobu Takao
    • 1
    • 2
  • Toshinori Okinaga
    • 1
  • Wataru Ariyoshi
    • 1
  • Kenjiro Iwanaga
    • 2
  • Ikuo Nakamichi
    • 3
  • Izumi Yoshioka
    • 4
  • Kazuhiro Tominaga
    • 2
    • 5
  • Tatsuji Nishihara
    • 1
    • 5
    Email author
  1. 1.Division of Infection and Molecular Biology, Department of Health PromotionKyushu Dental CollegeKitakyushuJapan
  2. 2.Division of Maxillofacial Diagnostic and Surgical Science, Department of Oral and Maxillofacial SurgeryKyushu Dental CollegeKitakyushuJapan
  3. 3.Division of General Internal Medicine, Department of Health PromotionKyushu Dental CollegeKitakyushuJapan
  4. 4.Department of Oral and Maxillofacial Surgery, Faculty of MedicineUniversity of MiyazakiKiyotake MiyazakiJapan
  5. 5.Oral Bioresearch CenterKyushu Dental CollegeKitakyushuJapan

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